A pair of oligonucleotide primers for the amplification of a target sequence between two primer binding sites located within the LTR region of the HIV-1 genome, said pair consisting of a first primer and a second primer, wherein said first primer consists essentially of a first oligonucleotide that is fully complementary to a sequence of the LTR region at a first primer binding site and binds thereto under conditions whereby nucleic acid amplification can occur, said oligonucleotide being 15-26 nucleotides in length and comprising at least 15 sequential nucleotides of the nucleotide sequence of:
G GGC GCC ACT GCT AGA GA;   SEQ ID NO:1 said first oligonucleotide being operably linked to a T3, T7, or SP6 promoter; and wherein said second primer consists essentially of comprises a second oligonucleotide that is fully complementary to a sequence which is the reverse complement of a sequence of the LTR region at a second primer binding site and binds thereto under conditions whereby nucleic acid amplification can occur, said oligonucleotide being 10-26 nucleotides in length and comprising at least 10 sequential nucleotides of the nucleotide sequence of:
CTC AAT AAA GCT TGC CTT GA   SEQ ID NO:5 wherein said primers amplify the target sequence between the two primer binding sites located within the LTR region of the HIV-1 genome when contacted with HIV-1 nucleic acid under conditions whereby nucleic acid amplification can occur. view more

The pair of oligonucleotide primers of claim 1, wherein said second oligonucleotide that is fully complementary to a sequence of the LTR region is 15-26 nucleotides in length and comprises at least 15 sequential nucleotides of the nucleotide sequence of:
CTC AAT AAA GCT TGC CTT GA.   SEQ ID NO:5 view more

A primer set for the amplification of a target sequence between two primer binding sites located within the LTR region of the HIV-1 genome, said set consisting of a first primer and a second primer, wherein said first primer consists essentially of a first hybridizing oligonucleotide that is fully complementary to a sequence of the LTR region at a first primer binding site and binds thereto under conditions whereby nucleic acid amplification can occur, said oligonucleotide being 15-26 nucleotides in length and comprising at least 15 sequential nucleotides of the nucleotide sequence of:
G GGC GCC ACT GCT AGA GA;   SEQ ID NO:1 said first hybridizing oligonucleotide being operably linked to a T3, T7, or SP6 promoter; and wherein said second primer consists essentially of a second hybridizing oligonucleotide that is fully complementary to a sequence which is the reverse complement of a sequence of the LTR region at a second primer binding site and binds thereto under conditions whereby nucleic acid amplification can occur, said oligonucleotide being 10-26 nucleotides in length and comprising at least 10 sequential nucleotides of the nucleotide sequence of:
CTC AAT AAA GCT TGC CTT GA   SEQ ID NO:5 wherein said primers amplify the target sequence between the two primer binding sites located within the LTR region of the HIV-1 genome when contacted with HIV-1 nucleic acid under conditions whereby nucleic acid amplification can occur. view more

The primer set of claim 3, wherein said second hybridizing oligonucleotide that is fully complementary to a sequence of the LTR region is 15-26 nucleotides in length and comprises at least 15 sequential nucleotides of the nucleotide sequence of:
CTC AAT AAA GCT TGC CTT GA.   SEQ ID NO:5 view more

A pair of oligonucleotide primers for the amplification of a target sequence between two primer binding sites located within the LTR region of the HIV-1 genome, said pair consisting of a first primer and a second primer, wherein said first primer consists essentially of comprises a first hybridizing oligonucleotide that is fully complementary to a sequence of the LTR region at a first primer binding site and binds thereto under conditions whereby nucleic acid amplification can occur, said oligonucleotide being 15-26 nucleotides in length, wherein said first hybridizing oligonucleotide comprises at least 15 sequential nucleotides of the nucleotide sequence of
G GGC GCC ACT GCT AGA GA;   SEQ ID NO:1 said hybridizing oligonucleotide being operably linked to a T3, T7, or SP6 promoter; and wherein said second primer consists essentially of a second hybridizing oligonucleotide that is fully complementary to a sequence which is the reverse complement of a sequence of the LTR region at a second primer binding site and binds thereto under conditions whereby nucleic acid amplification can occur, said oligonucleotide being 10-26 nucleotides in length, wherein said second hybridizing oligonucleotide comprises at least 10 sequential nucleotides of the nucleotide sequence of
CTC AAT AAA GCT TGC CTT GA   SEQ ID NO:5 wherein said primers amplify the target sequence between the two primer binding sites located within the LTR region of the HIV-1 genome when contacted with HIV-1 nucleic acid under conditions whereby nucleic acid amplification can occur. view more

The pair of oligonucleotide primers of claim 5, wherein said second hybridizing oligonucleotide that is fully complementary to a sequence of the LTR region is 15-26 nucleotides in length and comprises at least 15 sequential nucleotides of the nucleotide sequence of:
CTC AAT AAA GCT TGC CTT GA.   SEQ ID NO:5 view more