Methods for raising antibodies

• US 10,000,557 B2
• Filed: 12/18/2013
• Issued: 06/19/2018
• Est. Priority Date: 12/19/2012
• Status: Active Grant

• Current Assignee: DNAE Group Holdings Limited (Genting Bhd.)
• Original Assignee: DNAE Group Holdings Limited (Genting Bhd.)
• Inventors: Dykes, Colin, Dryga, Sergey A., Clarizia, Lisa-Jo Ann, Adams, Eddie W., Norvell, Meghan
• Primary Examiner(s): Weiler, Karen S.
• Application Number:
  US14/132,099
• Publication Number:
  US 20140171340A1
• Time in Patent Office: 1,644 Days
• Field of Search: None
• CPC Class Codes: C07K 16/12 against material from bacteria
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C40B 50/06 Biochemical methods, e.g. using enzymes or whole viable microorganisms
• CPC Class Codes: G16B 10/00 ICT specially adapted for evolutionary bioinformatics, e.g. phylogenetic tree construction or analysis
• CPC Class Codes: G16B 20/20 Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
• CPC Class Codes: G16B 20/40 Population genetics; Linkage disequilibrium

Abstract

The present invention generally relates to methods of generating antibodies against a species of pathogen that involve identifying the pathogen that is most genetically representative of member of pathogen species and using the identified pathogen to generate an antibody.

Claims

1. A method of generating antibodies against a species comprising pathogens, the method comprising:

obtaining nucleic acids from a plurality of pathogens within the species;

assaying said nucleic acids to determine genetic similarities between the pathogens by sequencing a portion of said nucleic acids by single molecule sequencing and comparing the sequences;

separating the pathogens into at least two different groups based upon the genetic similarities;

isolating at least one, but not all, pathogen from each group that exhibits representative characteristics of the group to form a subset of pathogens; and

generating antibodies for each isolated pathogen by administering each isolated pathogen to one or more mammals in an amount effective to produce an immune response in order to produce a set of antibodies.

2. The method of claim 1, wherein the nucleic acid is DNA.

3. The method of claim 1, wherein the pathogen comprise bacteria.

4. The method of claim 3, wherein the bacteria comprise bacteria of the same species but of different serotypes.

5. The method of claim 3, wherein the bacteria comprise bacteria of different strains.

6. The method of claim 1, wherein administering comprises injecting a mammal with each isolated pathogen.

7. The method of claim 6, wherein generating antibodies further comprises placing the pathogen in adjuvant prior to injecting the mammal.

8. The method of claim 1, wherein the antibodies are monoclonal antibodies.

9. The method of claim 1, wherein the antibodies are polyclonal antibodies.

10. The method of claim 1, wherein isolating the at least one pathogen comprises building a cladogram where genomically similar pathogens are grouped together to form clades.

11. The method of claim 1, wherein the subset of pathogens is representative of the genomic variation present in the species.

12. The method of claim 1, wherein the nucleic acid is RNA.