Methods and compositions for plant pest control

• US 10,000,767 B2
• Filed: 01/27/2014
• Issued: 06/19/2018
• Est. Priority Date: 01/28/2013
• Status: Expired due to Fees

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Crawford, Michael J., Li, Xiangqian, Kapoor, Mahak, Williams, Deryck Jeremy
• Primary Examiner(s): Visone, Lee A
• Assistant Examiner(s): Fan, Weihua
• Application Number:
  US14/165,097
• Publication Number:
  US 20140215656A1
• Time in Patent Office: 1,604 Days
• CPC Class Codes: A01H 1/1255 for fungal resistance
• CPC Class Codes: A01N 37/46 N-acyl derivatives
• CPC Class Codes: A01N 63/60 Isolated nucleic acids
• CPC Class Codes: A01N 65/00 Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof containing compounds of determined constitution A01N27/00 - A01N59/00
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8206 by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
• CPC Class Codes: C12N 15/8218 Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
• CPC Class Codes: C12N 15/8282 for fungal resistance
• CPC Class Codes: C12N 15/8285 for nematode resistance
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Provided are methods and compositions to improve fungal disease resistance in various crop plants. Also provided are combinations of compositions and methods to improve fungal disease resistance in various crop plants.

Claims

1. A method for producing a soybean plant exhibiting an improvement in Soy Cyst Nematode (SCN) resistance, the method comprising topically applying to a plant surface a composition that comprises:

(a) at least four polynucleotides that comprise;

(i) a polynucleotide of 25 to 150 nucleotides in length that comprises a segment of 25 contiguous nucleotides that are identical or complementary to SEQ ID NO;

126;

(ii) a polynucleotide of 25 to 150 nucleotides in length that comprises a segment of 25 contiguous nucleotides that are identical or complementary to SEQ ID NO;

127;

(iii) a polynucleotide of 25 to 150 nucleotides in length that comprises a segment of 25 contiguous nucleotides that are identical or complementary to SEQ ID NO;

128; and

(iv) a polynucleotide of 25 to 150 nucleotides in length that comprises a segment of 25 contiguous nucleotides that are identical or complementary to SEQ ID NO;

129,

wherein said polynucleotides are polynucleotide is not operably linked to a promoter or a viral vector and wherein said polynucleotides are not integrated into the plant chromosome; and

(b) a transfer agent; and

thereby producing a soybean plant that exhibits an improvement in SCN resistance resulting from suppression of a BAX inhibitor 1 (BI-1) gene.

2. The method of claim 1, wherein said polynucleotides comprise sense ssDNA, sense ssRNA, dsRNA, dsDNA, a double stranded DNA/RNA hybrid, anti-sense ssDNA, or anti-sense ssRNA.

3. The method of claim 1, wherein said composition further comprises a non-polynucleotide herbicidal molecule, a polynucleotide herbicidal molecule, a polynucleotide that suppresses an herbicide target gene, an insecticide, a fungicide, a nematocide, or a combination thereof.

4. The method of claim 1, wherein said composition further comprises a non-polynucleotide herbicidal molecule and said plant is resistant to said non-polynucleotide herbicidal molecule.

5. The method of claim 1, wherein said transfer agent comprises an organosilicone preparation.