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Isolation of polymerase-nucleic acid complexes

  • US 10,000,805 B2
  • Filed: 09/20/2016
  • Issued: 06/19/2018
  • Est. Priority Date: 03/23/2011
  • Status: Active Grant
First Claim
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1. A method for isolating DNA having a modified or unnatural base comprising:

  • obtaining a library of circular DNA fragments each comprising a double stranded DNA central region and single stranded regions on the ends of the double stranded regions wherein at least some of the fragments comprise one or more modified or unnatural base;

    treating the DNA fragments with a primer and a polymerase under conditions where the polymerase extends the primer to copy at least a portion of one strand of the double stranded region so as to render the other strand of the double-stranded portion single stranded;

    using a binding protein or antibody that is specific to the one or more modified or unnatural base on the portion of the circular DNA fragments that is rendered single stranded to isolate strands containing one or more modified or unnatural base from those that do not contain an unnatural or modified base.

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