Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
First Claim
Patent Images
1. A fluid sample processing cartridge comprising:
- a) a sample preparation module comprising;
i) a substrate;
ii) a sample well formed in said substrate and configured to receive a volume of fluid sample;
iii) a closure configured to be selectively placed over said sample well;
iv) a deformable fluid chamber supported on said substrate and configured to hold a fluid therein when in an undeformed state and to collapse upon application of an external compression force to expel at least a portion the fluid from the fluid chamber, said deformable fluid chamber being in fluid communication with said sample well via a channel formed in said substrate;
v) a mixing well formed in said substrate, said mixing well being in fluid communication with said sample well via a channel formed in said substrate; and
vi) a fluid exit port formed in the substrate, said fluid exit port being in fluid communication with said mixing well via a channel formed in said substrate; and
b) a reaction module attached to said sample preparation module and configured to receive a fluid from said sample preparation module via the fluid exit port formed in said sample preparation module, said reaction module comprising;
i) a top plate comprising1) a top surface;
2) a raised wall at least partially circumscribing said top surface and in fluid sealing contact with a surface of said sample preparation module to form an interstitial space between the top surface and the surface of said sample preparation module;
3) a sample chamber fluidly coupled to the fluid exit port of the sample preparation module;
4) a reagent chamber; and
5) a detection chamber; and
ii) a fluidic processing panel coupled to a bottom surface of said top plate and defining a reaction and processing space between said fluidic processing panel and said top plate, wherein said reaction and processing space is open or openable to said sample chamber, said reaction chamber, and said detection chamber, said fluidic processing panel comprising;
1) an electrowetting grid formed thereon, said electrowetting grid being configured for manipulation of fluid droplets within at least a portion of said reaction and processing space.
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Abstract
In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.
551 Citations
15 Claims
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1. A fluid sample processing cartridge comprising:
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a) a sample preparation module comprising; i) a substrate; ii) a sample well formed in said substrate and configured to receive a volume of fluid sample; iii) a closure configured to be selectively placed over said sample well; iv) a deformable fluid chamber supported on said substrate and configured to hold a fluid therein when in an undeformed state and to collapse upon application of an external compression force to expel at least a portion the fluid from the fluid chamber, said deformable fluid chamber being in fluid communication with said sample well via a channel formed in said substrate; v) a mixing well formed in said substrate, said mixing well being in fluid communication with said sample well via a channel formed in said substrate; and vi) a fluid exit port formed in the substrate, said fluid exit port being in fluid communication with said mixing well via a channel formed in said substrate; and b) a reaction module attached to said sample preparation module and configured to receive a fluid from said sample preparation module via the fluid exit port formed in said sample preparation module, said reaction module comprising; i) a top plate comprising 1) a top surface; 2) a raised wall at least partially circumscribing said top surface and in fluid sealing contact with a surface of said sample preparation module to form an interstitial space between the top surface and the surface of said sample preparation module; 3) a sample chamber fluidly coupled to the fluid exit port of the sample preparation module; 4) a reagent chamber; and 5) a detection chamber; and ii) a fluidic processing panel coupled to a bottom surface of said top plate and defining a reaction and processing space between said fluidic processing panel and said top plate, wherein said reaction and processing space is open or openable to said sample chamber, said reaction chamber, and said detection chamber, said fluidic processing panel comprising; 1) an electrowetting grid formed thereon, said electrowetting grid being configured for manipulation of fluid droplets within at least a portion of said reaction and processing space. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification