Multipotent adult stem cells and methods for isolation

• US 10,006,004 B2
• Filed: 04/01/2009
• Issued: 06/26/2018
• Est. Priority Date: 08/05/1999
• Status: Active Grant

• Current Assignee: ABT Holding Company (Athersys, Inc.)
• Original Assignee: ABT Holding Company (Athersys, Inc.)
• Inventors: Furcht, Leo T., Verfaillie, Catherine M., Reyes, Morayma
• Primary Examiner(s): Berke-Schlessel, David W
• Application Number:
  US12/416,715
• Publication Number:
  US 20090203130A1
• Time in Patent Office: 3,373 Days
• Field of Search: None
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 2239/31 characterized by the route of administration
• CPC Class Codes: A61K 39/461 characterised by the cell type used
• CPC Class Codes: A61K 39/46432 Nervous system antigens
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: A61P 37/06 Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: C12N 2501/105 Insulin-like growth factors [IGF]
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/135 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 2503/00 Use of cells in diagnostics
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 5/0607 Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696

Abstract

The invention provides isolated stem cells of non-embryonic origin that can be maintained in culture in the undifferentiated state or differentiated to form cells of multiple tissue types. Also provided are methods of isolation and culture, as well as therapeutic uses for the isolated cells.

Claims

1. A cell culture comprising isolated expanded human multipotent non-embryonic, non-germ cells that can differentiate into at least one cell type of at least two of the endodermal, ectodermal, and mesodermal embryonic lineages, said cells having undergone at least 10-40 cell doublings in culture.

2. The cell culture of claim 1 wherein the non-embryonic, non-germ cells express one or more of oct4, telomerase, rex-1, rox-1, or sox-2.

3. The cell culture of claim 1 wherein the non-embryonic, non-germ cells can differentiate into at least one cell type of each of the endodermal, ectodermal, and mesodermal embryonic lineages.

4. The cell culture of claim 3 wherein the non-embryonic, non-germ cells express one or more of oct4, telomerase, rex-1, rox-1, or sox-2.

5. A method for making a cell culture composition, said method comprising introducing the isolated expanded human non-embryonic, non-germ cells of claim 1 into cell culture medium.

6. A method for making the cell culture of claim 1, the method comprising:

a. obtaining the non-embryonic, non-germ cells from non-embryonic, non-germ tissue containing the cells, and

b. culturing the non-embryonic, non-germ cells to produce a cell culture in which the non-embryonic, non-germ cells have undergone 10-40 cell doublings.

7. A method for producing a differentiated progeny cell, said method comprising culturing the cells from the cell culture of claim 1 under conditions that are suitable to induce differentiation, thereby producing a differentiated progeny cell.

8. The cell culture as in any of claims 1-4, in which the isolated expanded human multipotent non-embryonic, non-germ cells are derived from bone marrow.

9. The method of claim 5 wherein the isolated expanded human multipotent non-embryonic, non-germ cells are derived from bone marrow.

10. The method of claim 6 wherein the isolated expanded human multipotent non-embryonic, non-germ cells are derived from bone marrow.