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Methods for control of flux in metabolic pathways through enzyme relocation

  • US 10,006,062 B2
  • Filed: 02/11/2015
  • Issued: 06/26/2018
  • Est. Priority Date: 05/07/2010
  • Status: Expired due to Fees
First Claim
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1. A bacterial cell that expresses enzymes of a biosynthetic pathway for production of a product, wherein at least one of the enzymes:

  • (i) controls metabolic flux in the biosynthetic pathway;

    (ii) is modified by genetic means to attach a periplasmic-targeting sequence; and

    (iii) is selected from the group consisting of amidophosphoribosyl transferase, orotate phosphoribosyltransferase, 2-dehydro-3-deoxyphosphoheptonate aldolase, phosphoribosyltransferase HisG, acetolactate/acetohydroxybutanoate synthase, UDP-N-acetylglucosamine acyltransferase, aspartate aminotransferase, arginine decarboxylase, GTP cyclohydrolase I, acetyl-CoA carboxylase, 2-keto-acid decarboxylase, acetyl-CoA acetyltransferase, and beta-ketothiolase,wherein the product is selected from the group consisting of antibiotics, biosurfactants, biological fuels, amino acids, organic acids, fatty acids, alcohols, polyols, flavors, fragrances, nucleotides, vitamins, pigments, sugars, polysaccharides, biopolymers, plastics, isoprenoids, terpenes, and cell metabolites.

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