Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array
First Claim
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1. An electrochemical deblocking solution comprising:
- an organic base including a lutidine, a benzoquinone and a hydroquinone;
tetraethylammonium p-toluenesulfonate as an organic salt; and
a solvent selected from the group consisting of an alcohol, acetonitrile and dichloromethane.
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Abstract
There is disclosed an electrochemical deblocking solution for use on an electrode microarray. There is further disclosed a method for electrochemical synthesis on an electrode array using the electrochemical deblocking solution. The solution and method are for removing acid-labile protecting groups for synthesis of oligonucleotides, peptides, small molecules, or polymers on a microarray of electrodes while substantially improving isolation of deblocking to active electrodes. The method comprises applying a voltage or a current to at least one electrode of an array of electrodes. The array of electrodes is covered by the electrochemical deblocking solution.
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Citations
20 Claims
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1. An electrochemical deblocking solution comprising:
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an organic base including a lutidine, a benzoquinone and a hydroquinone; tetraethylammonium p-toluenesulfonate as an organic salt; and a solvent selected from the group consisting of an alcohol, acetonitrile and dichloromethane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of electrochemical deblocking an oligomer comprising the steps of:
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covering an array of electrodes with a deblocking solution including; lutidine, benzoquinone, hydroquinone and tetraethylammonium p-toluenesulfonate; and one or more solvents selected from the group consisting of an alcohol, acetonitrile and dichloromethane; and applying one or both a voltage and a current to at least one electrode of the array of electrodes. - View Dependent Claims (16, 17, 18, 19)
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20. A kit for electrochemical deblocking comprising:
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an array of electrodes adapted to connect with one or both a voltage and a current source to apply one or both a voltage and a current to at least one electrode of the array of electrodes; a deblocking solution including; lutidine, benzoquinone, hydroquinone and tetraethylammonium p-toluenesulfonate; and one or more solvents selected from the group consisting of an alcohol, acetonitrile and dichloromethane; and instructions for one or both preparing the deblocking solution and applying one or both a voltage and a current to at least one electrode of the array of electrodes.
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Specification