Devices for real-time polymerase chain reaction

• US 10,006,861 B2
• Filed: 06/30/2014
• Issued: 06/26/2018
• Est. Priority Date: 06/28/2013
• Status: Active Grant

• Current Assignee: Streck
• Original Assignee: Streck
• Inventors: Kreifels, Matthew R., Whitney, Scott E., Wilder, Gregg, Flanagan, John, Dowling, James, Hills, Philip, Tilleman, Michael, Jackson, Jeremie
• Primary Examiner(s): Bowers, Nathan A
• Assistant Examiner(s): Edwards, Lydia
• Application Number:
  US14/911,671
• Publication Number:
  US 20160202184A1
• Time in Patent Office: 1,457 Days
• Field of Search: 4352872
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2561/113 Real time assay
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous

Abstract

An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate detection of stationary samples, reduced sample read time, and simultaneous reading of multiple light wavelengths.

Claims

1. An instrument for performing polymerase chain reaction with real-time detection comprising:

a) a sample holder configured to receive a plurality of sample tubes that each have at least one portion that is generally optically transparent, and that receives a biological sample having a nucleic acid to be amplified and at least one fluorescing agent that interacts with the nucleic acid during amplification and that emits light upon excitation by light of a known wavelength;

b) at least one light emitting diode device that is carried on at least one support substrate, is in electrical communication with a power source, and is adapted to emit light at a plurality of different wavelengths;

c) at least one photodiode array detector adapted to issue signals based upon intensity of light it receives;

d) a plurality of fiber optic bundles including a set of excitation fiber optic bundles and a set of emission fiber optic bundles;

e) a filter wheel including at least one filter pair, wherein the at least one filter pair includes;

i) an emission filter employed between the plurality of sample tubes and the at least one photodiode array detector;

ii) an excitation filter employed between the at least one light emitting diode device and the set of excitation fiber optic bundles; and

f) a plurality of sample alignment ports at a bottom of the sample holder which align the plurality of fiber optic bundles underneath the plurality of sample tubes.

2. The instrument of claim 1, wherein the instrument is free of any filter that is both the emission filter and the excitation filter.

3. The instrument of claim 1, wherein each filter allows two or more bands of light wavelengths to pass.

4. The instrument of claim 1, wherein the instrument is free of any filters that allow only a single hand of light wavelength to pass.

5. The instrument of claim 1, wherein one of each filter pair of the at least one filter pair includes one filter that is exclusively the emission filter and one filter that is exclusively the excitation filter.

6. The instrument of claim 1, wherein the instrument is free of any filters that are not exclusively the emission filters or the excitation filter.

7. The instrument of claim 1, wherein a number of filters matches exactly a number of fluorophore classes detected by the instrument.

8. The instrument of claim 1, wherein the instrument is free of any filters that must align with both an emission light path and an excitation light path.

9. The instrument of claim 1, wherein one or more filters do not align with both an emission light path and an excitation light path.

10. The instrument of claim 1, wherein all filters do not align with both an emission light path and an excitation light path.

11. The instrument of claim 1, including the at least one light emitting diode device having at least four light emitting diodes.

12. The instrument of claim 1, wherein the at least one light emitting diode device is located within a light source packaging that is less than 1 cm along each side.

13. The instrument of claim 1, including a light check photodiode detector to monitor a light output of each light source.

14. The instrument of claim 1, wherein a distance between a center of the at least one light emitting diode device and a center of the at least one photodiode array detector is 5.5 cm or less.

15. The instrument of claim 1, wherein some of the light emitted from at least one fluorophore in a sample will pass through the plurality of sample alignment ports and the set of emission fiber optic bundles carries the emitted light through a fiber optic manifold.

16. The instrument of claim 1, wherein the plurality of sample alignment ports align the plurality of fiber optic bundles underneath the plurality of sample tubes so light exits the plurality of fiber optic bundles along a vertical axis of each sample tube.

17. The instrument of claim 1, including at least one doublet excitation lens and at least one doublet emission lens.

18. The instrument of claim 1, wherein the at least one light emitting diode device has at least two light emitting diodes.

19. An instrument for performing polymerase chain reaction with real-time detection comprising:

a) a sample holder configured to receive a plurality of sample tubes that each have at least one portion that is generally optically transparent, and that receives a biological sample having a nucleic acid to be amplified and at least one fluorescing agent that interacts with the nucleic acid during amplification and that emits light upon excitation by light of a known wavelength;

b) at least one light emitting diode device that is carried on at least one support substrate, is in electrical communication with a power source, and is adapted to emit light at a plurality of different wavelengths;

c) at least one photodiode array detector adapted to issue signals based upon intensity of light it receives;

d) a plurality of fiber optic bundles including a set of excitation fiber optic bundles and a set of emission fiber optic bundles;

e) excitation doublet lenses employed in-line between the at least one light emitting diode device and the set of excitation fiber optic bundles, wherein the excitation doublet lenses include;

i) a first excitation doublet lens configured to approximately collimate along an axis of an excitation light path;

ii) a second excitation doublet lens configured to refocus the light onto an excitation plate;

f) emission doublet lenses employed in-line between the set of emission fiber optic bundles and the photodiode array detector, wherein the emission doublet lenses include;

i) a first emission doublet lens configured to approximately collimate along an axis of an emission light path;

ii) a second emission doublet lens configured to refocus the light onto the photodiode array detector;

g) a filter wheel including at least one filter pair, wherein the at least one filter pair includes;

i) an emission filter employed between the plurality of sample tubes and the at least one photodiode array detector;

ii) an excitation filter employed between the at least one light emitting diode device and the set of excitation fiber optic bundles; and

h) a plurality of sample alignment ports at a bottom of the sample holder which align the plurality of fiber optic bundles underneath the plurality of sample tubes.

20. The instrument of claim 19, wherein one of each filter pair of the at least one filter pair includes one filter that is exclusively the emission filter and one filter that is exclusively the excitation filter.