Raman spectroscopy for detection of glycated analytes
First Claim
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1. A method for detecting of one or more analytes in a sample using Raman shifted light, comprising the steps of:
- obtaining a solution including the sample from a patient, the sample comprising a glycated analyte;
concentrating the sample from the patient to form a concentrated sample;
illuminating the concentrated sample with light from a light source such that at least a portion of the sample emits Raman shifted light;
detecting the Raman shifted light and generating Raman spectral data for the glycated analyte in the concentrated sample; and
analyzing the Raman spectral data to determine a level of at least the glycated analyte in the sample.
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Abstract
The present invention relates to the optical measurement of blood analytes, such as glycated hemoglobin (HbA1c) and serum albumin as a functional metric of mean blood glucose in the diagnosis of diabetic patients. Non-enhanced Raman spectroscopy is employed as the analytical method for quantitative detection of blood analytes. Using processing techniques, non-enzymatic glycosylation (glycation) of the analytes results in measurable and highly reproducible changes in the acquired spectral data, which enable the accurate measurements and classification of glycated and unglycated analytes.
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Citations
39 Claims
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1. A method for detecting of one or more analytes in a sample using Raman shifted light, comprising the steps of:
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obtaining a solution including the sample from a patient, the sample comprising a glycated analyte; concentrating the sample from the patient to form a concentrated sample; illuminating the concentrated sample with light from a light source such that at least a portion of the sample emits Raman shifted light; detecting the Raman shifted light and generating Raman spectral data for the glycated analyte in the concentrated sample; and analyzing the Raman spectral data to determine a level of at least the glycated analyte in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A system for detecting a glycated analyte in a sample using Raman shifted light comprising:
- a sample processing concentrator device to concentrate a sample from a patient that includes a glycated analyte;
a light delivery system to illuminate the concentrated sample with light;
a detector that detects Raman shifted light from the glycated analyte in the concentrated sample, the detector generating Raman spectral data; and
a data processor that processes the Raman spectral data, the data processor being programmed with instructions to determine a quantitative concentration level of at least the glycated analyte in the sample. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28, 29)
- a sample processing concentrator device to concentrate a sample from a patient that includes a glycated analyte;
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30. A method for simultaneous detection of glycated hemoglobin and glycated serum albumin comprising the steps of:
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obtaining a solution including a sample from a patient, the sample comprising hemoglobin and serum albumin; depositing a portion of the solution onto a surface and drying the solution to form a dried concentrated sample; acquiring Raman spectral data from the dried sample; and analyzing the Raman spectral data to determine levels of glycated hemoglobin and serum albumin. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39)
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Specification