System and method for processing and detecting nucleic acids
First Claim
1. A system for processing nucleic acid content of a sample in cooperation with a cartridge comprising a heating region defined at a first surface, and a magnet housing region defined by a second surface in thermal communication with the first surface, the system comprising:
- a molecular diagnostic module comprising;
a cartridge platform comprising a magnet receiving slot and operable to receive the cartridge and align the second surface of the cartridge with the magnet receiving slot,a heater operable to transmit heat to the heating region of the cartridge at the first surface, anda magnet coupled to a magnet heating element that heats the magnet, the magnet operable to pass through the magnet receiving slot into the magnet housing region, thereby facilitating separation of a nucleic acid volume from the sample and heating the sample in cooperation with the heater.
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Accused Products
Abstract
A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids.
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Citations
20 Claims
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1. A system for processing nucleic acid content of a sample in cooperation with a cartridge comprising a heating region defined at a first surface, and a magnet housing region defined by a second surface in thermal communication with the first surface, the system comprising:
a molecular diagnostic module comprising; a cartridge platform comprising a magnet receiving slot and operable to receive the cartridge and align the second surface of the cartridge with the magnet receiving slot, a heater operable to transmit heat to the heating region of the cartridge at the first surface, and a magnet coupled to a magnet heating element that heats the magnet, the magnet operable to pass through the magnet receiving slot into the magnet housing region, thereby facilitating separation of a nucleic acid volume from the sample and heating the sample in cooperation with the heater. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for processing and detecting nucleic acids from a sample with a cartridge including a fluidic pathway passing between a heating region of the cartridge and a magnet housing region of the cartridge, wherein the method comprises:
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with a liquid handling system, combining the sample with a quantity of magnetic beads to produce a nucleic acid-magnetic bead sample; with the liquid handling system, transferring the nucleic acid-magnetic bead sample to the fluidic pathway of the cartridge; and contemporaneously transmitting heat through the heating region, by way of a heater, and transmitting heat through the magnet housing region, by way of a magnet heating element coupled to a magnet, thereby facilitating capture of nucleic acids of the sample at the fluidic pathway of the cartridge. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification