Immunological reagents and uses therefor

• US 10,011,602 B2
• Filed: 07/05/2013
• Issued: 07/03/2018
• Est. Priority Date: 07/06/2012
• Status: Active Grant

• Current Assignee: Monash University, The University of Queensland, University of Melbourne
• Original Assignee: University of Melbourne
• Inventors: Kjer-Nielsen, Lars, McCluskey, James, Corbett, Alexandra, Rossjohn, Jamie, Onisha, Patel, Fairlie, David Paul, Liu, Ligong
• Primary Examiner(s): Reddig, Peter J
• Application Number:
  US14/413,164
• Publication Number:
  US 20150166542A1
• Time in Patent Office: 1,824 Days
• Field of Search: None
• CPC Class Codes: A61K 38/1774 Immunoglobulin superfamily (e.g. CD2, CD4, CD8, ICAM molecules, B7 molecules, Fc-receptors, MHC-molecules)
• CPC Class Codes: C07D 475/02 with an oxygen atom directly attached in position 4
• CPC Class Codes: C07D 475/04 with a nitrogen atom directly attached in position 2
• CPC Class Codes: C07K 14/70539 MHC-molecules, e.g. HLA-molecules
• CPC Class Codes: C07K 2299/00 Coordinates from 3D structures of peptides, e.g. proteins or enzymes
• CPC Class Codes: C12N 2500/46 Amines, e.g. putrescine
• CPC Class Codes: C12N 5/0636 T lymphocytes
• CPC Class Codes: G01N 2333/70539 MHC-molecules, e.g. HLA-molecules
• CPC Class Codes: G01N 33/505 involving T-cells
• CPC Class Codes: G01N 33/56972 White blood cells

Abstract

The present invention provides a ligand which binds to MR1 wherein said binding results in binding of the MR1 to MAIT cells.

Claims

1. A method of detecting the presence of MAIT cells in a biological sample from a subject, the method comprising the steps of

a) contacting the biological sample with antigen presenting cells expressing MR1 bound to a ligand or a soluble form of MR1 bound to a ligand, under conditions that would allow binding of the MR1 or the soluble form of MR1 with MATT cells present in the sample, wherein the ligand is a compound selected from the group consisting of rRL-6HM, rRL-6AM, RL-6M, RP-5PA, 6-formyl pterin, acetyl 6-formyl pterin, 6-methyl-7-hydroxy-8-ribityl lumazine, and 6-,7-dimethyl-8-Ribityl Lumazine;

and

b) detecting the presence of MAIT cell bound MR1 or MAIT cell bound soluble form of MR1 in the biological sample,

thereby detecting the presence of MAIT cells in a biological sample from a subject.

2. The method according to claim 1, wherein the ligand is rRL-6HM.

3. The method of claim 1, wherein the biological sample is a biological fluid.

4. The method of claim 1, wherein the biological sample is a cell sample.

5. The method of claim 1, wherein the biological sample is a tissue sample.

6. The method of claim 3, wherein the biological fluid is serum, lymph, or blood.

7. The method of claim 5, wherein the tissue sample is a bone marrow or a tissue biopsy sample including mucosal tissue.

8. The method of claim 7, wherein the tissue biopsy sample is from the gut, gut lamina propria, or lungs.

9. The method of claim 1, wherein the MR1 is a human, primate, or mouse MR1 polypeptide.

10. The method of claim 8, wherein the MR1 polypeptide comprises SEQ ID NO:

1 or SEQ ID NO;

4.

11. The method of claim 1, wherein the subject is a human.