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Cellular libraries of peptide sequences (CLiPS) and methods of using the same

  • US 10,017,757 B2
  • Filed: 07/21/2015
  • Issued: 07/10/2018
  • Est. Priority Date: 08/31/2005
  • Status: Active Grant
First Claim
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1. A peptide display scaffold comprising a fusion protein comprising the formula:


  • [D1-C1]-TM-[D2],wherein TM is a circularly permuted transmembrane protein;

    wherein C1 comprises a candidate peptide;

    wherein D1 is a first detectable moiety and is a heterologous peptide or polypeptide;

    wherein D2 is a second detectable moiety and is a heterologous peptide or polypeptide;

    wherein D1 and D2 are not the same; and

    wherein D1, D2, and C1, when the peptide display scaffold is expressed in a host cell, are exposed at an extracellular surface of the host cell outer membrane,wherein the circularly permuted transmembrane protein is a circularly permuted OmpX, OmpA, or OmpT.

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