Cellular libraries of peptide sequences (CLiPS) and methods of using the same
First Claim
Patent Images
1. A peptide display scaffold comprising a fusion protein comprising the formula:
-
[D1-C1]-TM-[D2],wherein TM is a circularly permuted transmembrane protein;
wherein C1 comprises a candidate peptide;
wherein D1 is a first detectable moiety and is a heterologous peptide or polypeptide;
wherein D2 is a second detectable moiety and is a heterologous peptide or polypeptide;
wherein D1 and D2 are not the same; and
wherein D1, D2, and C1, when the peptide display scaffold is expressed in a host cell, are exposed at an extracellular surface of the host cell outer membrane,wherein the circularly permuted transmembrane protein is a circularly permuted OmpX, OmpA, or OmpT.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides compositions including peptide display scaffolds that present at least one candidate peptide and at least one detectable moiety in at least one of the N-terminal and C-terminal candidate peptide presenting domains that when expressed in a cell are accessible at a surface of the cell outermembrane. In addition, the present invention also provides kits and methods for screening a library of cells presenting the candidate peptides in peptide display scaffolds to identify a ligand for an enzyme.
-
Citations
29 Claims
-
1. A peptide display scaffold comprising a fusion protein comprising the formula:
-
[D1-C1]-TM-[D2],wherein TM is a circularly permuted transmembrane protein; wherein C1 comprises a candidate peptide; wherein D1 is a first detectable moiety and is a heterologous peptide or polypeptide; wherein D2 is a second detectable moiety and is a heterologous peptide or polypeptide; wherein D1 and D2 are not the same; and wherein D1, D2, and C1, when the peptide display scaffold is expressed in a host cell, are exposed at an extracellular surface of the host cell outer membrane, wherein the circularly permuted transmembrane protein is a circularly permuted OmpX, OmpA, or OmpT. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
-
Specification