Methods and compositions for sequencing nucleic acids using charge
First Claim
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1. A method for sequencing nucleic acids comprising,a) incorporating one or more nucleotides into a plurality of nucleic acids in one or more reaction chambers in contact with one or more charge detectors, wherein said nucleotides comprise a cleavable label and a 3′
- -OH blocking group,b) cleaving said cleavable label under conditions such that a charged moiety is produced, wherein said charged moiety is a positively charged moiety or a negatively charged moiety, the nature of the charge being correlated with the identity of the base; and
c) detecting said charged moiety with said one or more charge detectors under conditions such that said one or more nucleotides are identified, wherein said positively charged moiety identifies a pyrimidine nucleotide.
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Abstract
The invention relates to methods and compositions, and systems for determining the identity of nucleic acids in nucleotide sequences, and in particular, sequences that contain consecutive repeats of a particular base. For example, a consecutive repeat of a particular base may be identified by a charged ion detection (e.g., hydrogen ion detection).
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12 Claims
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1. A method for sequencing nucleic acids comprising,
a) incorporating one or more nucleotides into a plurality of nucleic acids in one or more reaction chambers in contact with one or more charge detectors, wherein said nucleotides comprise a cleavable label and a 3′ - -OH blocking group,
b) cleaving said cleavable label under conditions such that a charged moiety is produced, wherein said charged moiety is a positively charged moiety or a negatively charged moiety, the nature of the charge being correlated with the identity of the base; and c) detecting said charged moiety with said one or more charge detectors under conditions such that said one or more nucleotides are identified, wherein said positively charged moiety identifies a pyrimidine nucleotide. - View Dependent Claims (2, 3, 4, 5, 6)
- -OH blocking group,
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7. A method for sequencing nucleic acids comprising,
a) incorporating one or more nucleotides into a plurality of nucleic acids in one or more reaction chambers in contact with one or more charge detectors, wherein said nucleotides comprise a cleavable label and a 3′ - -OH blocking group,
b) cleaving said cleavable label under conditions such that a charged moiety is produced, wherein said charged moiety is a positively charged moiety or a negatively charged moiety, the nature of the charge being correlated with the identity of the base; and c) detecting said charged moiety with said one or more charge detectors under conditions such that said one or more nucleotides are identified, wherein said negatively charged moiety identifies a purine nucleotide. - View Dependent Claims (8, 9, 10, 11, 12)
- -OH blocking group,
Specification