Optical imaging system and methods for using the same
First Claim
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1. A system for assaying a sample for an analyte, the system comprising:
- a broad spectrum light source;
a slit projection module coupled to the broad spectrum light source, wherein the slit projection module comprises;
a slit that narrows a beam of light from the broad spectrum light source to a width equal to the width of the slit; and
a focusing lens that focuses light from the slit;
an objective lens which focuses light transmitted from a sample;
a diffraction grating; and
a detector for detecting one or more predetermined wavelengths of the transmitted light,wherein the light source, slit projection module, objective lens, diffraction grating and detector are positioned in line with each other such that light from the broad spectrum light source is propagated along an optical axis through the slit and focusing lens and is transmitted through the sample, the objective lens and the diffraction grating directly to the detector without substantial diversion from the optical axis.
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Abstract
Aspects of the present disclosure include methods and systems for assaying a sample for an analyte. Methods according to certain embodiments include illuminating a sample with a slit-shaped beam of light, detecting light transmitted through the sample, determining absorbance of the transmitted light at one or more wavelengths and calculating concentration of the analyte based on the absorbance to assay the sample for the analyte. Systems for practicing the subject methods are also described.
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Citations
22 Claims
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1. A system for assaying a sample for an analyte, the system comprising:
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a broad spectrum light source; a slit projection module coupled to the broad spectrum light source, wherein the slit projection module comprises; a slit that narrows a beam of light from the broad spectrum light source to a width equal to the width of the slit; and a focusing lens that focuses light from the slit; an objective lens which focuses light transmitted from a sample; a diffraction grating; and a detector for detecting one or more predetermined wavelengths of the transmitted light, wherein the light source, slit projection module, objective lens, diffraction grating and detector are positioned in line with each other such that light from the broad spectrum light source is propagated along an optical axis through the slit and focusing lens and is transmitted through the sample, the objective lens and the diffraction grating directly to the detector without substantial diversion from the optical axis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 21)
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18. A system comprising:
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a broad spectrum light source; a slit projection module coupled to the broad spectrum light source, wherein the slit projection module comprises; a slit that narrows a beam of light from the broad spectrum light source to a width equal to the width of the slit; and a focusing lens that focuses light from the slit; a cartridge holder configured to receive a microfluidic device having a capillary channel sample chamber; a diffraction grating; and a detector for detecting one or more predetermined wavelengths of the transmitted light; and a microfluidic device configured to perform an assay of a liquid sample positioned in the cartridge holder , the device comprising; a sample application site in fluid communication with an inlet into a capillary channel sample chamber; a capillary channel sample chamber; and a reagent mixing chamber positioned between the sample application site and the capillary channel sample chamber, wherein the light source, slit projection module, objective lens, diffraction grating and detector are positioned in line with each other such that light from the broad spectrum light source is propagated along an optical axis through the slit and focusing lens and is transmitted through the sample, the objective lens and the diffraction grating directly to the detector without substantial diversion from the optical axis. - View Dependent Claims (20, 22)
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Specification