Methods and devices for reading microarrays
First Claim
Patent Images
1. A method for improving image flatness of a surface image of a probe array having an array surface roughness, the method comprising:
- imaging one or more fiducials of the probe array at a plurality of positions along an axis of translation and determining a best position measurement for each of the fiducials at which the imaging is sharpest;
generating a surface fit profile based on a plurality of the best position measurements; and
imaging the probe array and adjusting one or more surface non-flatness parameters based on the surface fit profile to improve the image flatness of the surface image of the probe array.
1 Assignment
0 Petitions
Accused Products
Abstract
In one embodiment of the invention, a method to image a probe array is described that includes focusing on a plurality of fiducials on a surface of an array. The method utilizes obtaining the best z position of the fiducials and using a surface fitting algorithm to produce a surface fit profile. One or more surface non-flatness parameters can be adjusted to improve the flatness image of the array surface to be imaged.
-
Citations
21 Claims
-
1. A method for improving image flatness of a surface image of a probe array having an array surface roughness, the method comprising:
-
imaging one or more fiducials of the probe array at a plurality of positions along an axis of translation and determining a best position measurement for each of the fiducials at which the imaging is sharpest; generating a surface fit profile based on a plurality of the best position measurements; and imaging the probe array and adjusting one or more surface non-flatness parameters based on the surface fit profile to improve the image flatness of the surface image of the probe array. - View Dependent Claims (2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
4. The method of 1, wherein determining the best position measurement comprises using quadratic interpolation for at least one of the fiducials.
Specification