Mesenchymal stem cell extract and its use

• US 10,022,313 B2
• Filed: 07/02/2014
• Issued: 07/17/2018
• Est. Priority Date: 02/17/2014
• Status: Active Grant

• Current Assignee: Gwo Xi Stem Cell Applied Technology Co., Ltd.
• Original Assignee: Gwo Xi Stem Cell Applied Technology Co., Ltd.
• Inventors: Ling, Shinn-Zong, Harn, Horng-Jyh, Chiou, Tzyy-Wen, Lin, Po-Cheng
• Primary Examiner(s): Long, Scott
• Assistant Examiner(s): Nguyen, Nghi V
• Application Number:
  US14/321,954
• Publication Number:
  US 20150231052A1
• Time in Patent Office: 1,476 Days
• Field of Search: None
• CPC Class Codes: A61F 2007/0003 Face
• CPC Class Codes: A61F 2007/0062 the gas being steam or water vapour
• CPC Class Codes: A61F 2007/0088 Radiating heat
• CPC Class Codes: A61F 7/02 Compresses or poultices for effecting heating or cooling
• CPC Class Codes: A61K 2800/805 Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
• CPC Class Codes: A61K 2800/86 Products or compounds obtained by genetic engineering
• CPC Class Codes: A61K 2800/88 Two- or multipart kits
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/981 of mammals or bird
• CPC Class Codes: A61N 5/0625 Warming the body, e.g. hyperthermia treatment
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/14 for baldness or alopecia
• CPC Class Codes: A61Q 19/08 Anti-ageing preparations
• CPC Class Codes: A61Q 7/00 Preparations for affecting hair growth

Abstract

A mesenchymal stem cell extract and its use are provided, wherein the mesenchymal stem cell extract comprises a trophic factor(s), such as bone morphogenetic protein-7 (BMP-7), stromal cell-derived factor-1 (SDF-1), vascular endothelial growth factor (VEGF), C-X-C chemokine receptor type-4 (CXCR4), brain-derived neurotrophic factor (BDNF), and/or interleukin-17 (IL-17), and wherein the extract is especially suitable for repairing skin aging.

Claims

1. A method for at least one of promoting the expression of hyaluronic acid (HA), elastin, collagen and/or reticulin in the skin, stimulating cell proliferation at the administered region, stimulating cells at the administered region to secrete a trophic factor, and stimulating endogenous hematopoietic progenitor cells (HPC) to gather and proliferate at the administered region in a subject, comprising administering to the subject an effective amount of a mesenchymal stem cell extract, wherein the mesenchymal stern cell extract is a supernatant comprising bone morphogenetic protein-7 (BMP-7), stromal cell-derived factor-1 (SDF- 1), vascular endothelial growth factor (VEGF), C-X-C chemokine receptor type-4 (CXCR4), brain-derived neurotrophic factor (BDNF) and interleukin-17 (IL-17), and the supernatant is provided by the following steps:

(1) mixing mesenchymal stem cells separated from an animal fat tissue with a proteolysis buffer containing NP40 lysis buffer, phenylmethanesulfonyl fluoride (PMSF), a protease inhibitor, p-glycerol phosphate and Na3V04, to lyse the cells and provide a cell lysate; and

b(2) centrifuging the cell lysate at a temperature of no more than 4°

C. to collect the supernatant.

2. The method as claimed in claim 1, which is for repairing skin aging.

3. The method as claimed in claim 2, wherein the mesenchymal stem cell extract is administered as an emulsion, a cream, a gel, a paste, a spray or a solution.

4. The method as claimed in claim 3, wherein the gel is an aquagel, the paste is a dispersing paste or an ointment, and the solution is a washing fluid or a suspension.

5. The method as claimed in claim 2, wherein the mesenchymal stem cell extract is applied to the skin surface of the subject.

6. The method as claimed in claim 2, further comprising heating the skin surface with a heating means simultaneously with or separately from applying the mesenchymal stem cell extract.

7. The method as claimed in claim 6, wherein the heating means is at least one of a contact heating means and a non-contact heating means.

8. The method as claimed in claim 7, wherein the contact heating means is at least one of a facial mask, a heating pack, a hot towel, a heating pad, and a heating plate.

9. The method as claimed in claim 7, wherein the non-contact heating means is at least one of a steam and a heating lamp irradiation.

10. The method as claimed in claim 2, which is further for at least one of reducing hair loss, promoting the activation of hair follicle cells, promoting the proliferation of hair follicle cells, and promoting hair growth.