×

Methods to regulate polarization and enhance function of cells

  • US 10,022,457 B2
  • Filed: 06/17/2015
  • Issued: 07/17/2018
  • Est. Priority Date: 08/05/2005
  • Status: Active Grant
First Claim
Patent Images

1. A method for at least one of editing or regulating a gene in a target excitable cell, the method comprisingadministering to a patient in need thereof a plurality of nanoparticles coated with a biocompatible molecule for cell uptake, the nanoparticles conjugated with at least one opsin family gene, a second unmutated gene other than the opsin family gene which ameliorates a genetic or acquired degenerative disease or condition, an antibody that targets the nanoparticles to the target excitable cell, and a CRISPR/cas9 complex to form a complex of coated nanoparticle-gene-CRISPR/cas9;

  • delivering the nanoparticle-gene-CRISPR/cas9 complex to the target cell using the nanoparticle of the complex as a carrier without using a viral vector, and stimulating the complex and the target excitable cell with one or more energy sources under conditions sufficient to introduce the at least one opsin-family gene, the second gene, and the CRISPR/cas9 complex into the target excitable cell;

    using the CRISPR/cas9 complex to excise from the DNA of the target excitable cell a DNA segment bearing a mutation inducing the genetic or acquired degenerative disorder or condition; and

    replacing the excised mutated DNA segment with the second unmutated gene to treat the patient;

    wherein stimulating the complex and the target excitable cell with one or more energy sources includes stimulating the nanoparticle of the complex and the target excitable cell with light.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×