Method for removal of viruses from blood by lectin affinity hemodialysis
First Claim
1. A lectin affinity hemodialysis cartridge, comprising:
- a filtration chamber configured to receive blood or plasma contaminated with viral particles;
a lectin covalently coupled to agarose or to aminocelite disposed within said filtration chamber; and
a porous hollow fiber membrane, wherein said membrane has pores of 200-500 nm in diameter;
wherein the lectin is selected from the group consisting of;
Galanthus nivalis agglutinin (GNA), Narcissus pseudonarcissus agglutinin (NPA), cyanovirin, and Conconavalin A, and mixtures thereofwherein the cartridge is configured to remove said viral particles from the blood or plasma.
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Abstract
The present invention relates to a method for using lectins that bind to pathogens having high mannose surface glycoproteins or fragments thereof which contain high mannose glycoproteins, to remove them from infected blood or plasma in an extracorporeal setting. Accordingly, the present invention provides a method for reducing viral load in an individual comprising the steps of obtaining blood or plasma from the individual, passing the blood or plasma through a porous hollow fiber membrane wherein lectin molecules are immobilized within the porous exterior portion of the membrane, collecting pass-through blood or plasma and reinfusing the pass-through blood or plasma into the individual.
46 Citations
5 Claims
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1. A lectin affinity hemodialysis cartridge, comprising:
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a filtration chamber configured to receive blood or plasma contaminated with viral particles; a lectin covalently coupled to agarose or to aminocelite disposed within said filtration chamber; and a porous hollow fiber membrane, wherein said membrane has pores of 200-500 nm in diameter; wherein the lectin is selected from the group consisting of;
Galanthus nivalis agglutinin (GNA), Narcissus pseudonarcissus agglutinin (NPA), cyanovirin, and Conconavalin A, and mixtures thereofwherein the cartridge is configured to remove said viral particles from the blood or plasma. - View Dependent Claims (2, 3, 4, 5)
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Specification