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Microorganisms genetically engineered to have modified N-glycosylation activity

  • US 10,023,854 B2
  • Filed: 09/14/2012
  • Issued: 07/17/2018
  • Est. Priority Date: 04/03/2007
  • Status: Active Grant
First Claim
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1. A method of producing an altered N-glycosylation form of a target protein, the method comprising:

  • providing a Yarrowia lipolytica or an Arxula adeninivorans cell genetically engineered to express a protein capable of promoting mannosyl phosphorylation of N-glycans; and

    introducing into the cell a nucleic acid encoding a target protein, wherein the cell produces the target protein in an altered N-glycosylation form, andwherein the protein capable of promoting mannosyl phosphorylation of N-glycans is selected from the group consisting of;

    a wild-type MNN4 polypeptide or a biologically active variant of a wild-type MNN4 polypeptide;

    a wild-type PNO1 polypeptide or a biologically active variant of a wild-type PNO1 polypeptide; and

    a wild-type MNN6 polypeptide.

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