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Analysis of dried blood spot samples in a microfluidic system with dilution of extracted samples

  • US 10,024,826 B2
  • Filed: 08/22/2012
  • Issued: 07/17/2018
  • Est. Priority Date: 08/22/2011
  • Status: Active Grant
First Claim
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1. A chromatography apparatus comprising:

  • a first microfluidic substrate having a first fluidic channel comprising an analytical chromatographic column and a first fluidic port on one side of the first microfluidic substrate, the first fluidic port opening at a head end of the analytical chromatographic column; and

    a second microfluidic substrate having a second fluidic channel comprising a sample-preparation column, a second fluidic port on a first side of the second microfluidic substrate and opening into an end of the sample-preparation column, the sample-preparation column terminating at the head end of the analytical column, and a third fluidic port on a second side of the second microfluidic substrate;

    a dried blood spot (DBS) collection device holding one or more dried biological samples, the DBS collection device being directly coupled to the second microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the second fluidic channel of the microfluidic substrate such that the second fluidic port of the second microfluidic substrate receives an extraction of that biological sample and passes the extracted biological sample into the sample-preparation column;

    wherein the second side of the second microfluidic substrate is coupled to the first microfluidic substrate such that the third fluidic port of the second microfluidic substrate is in fluidic communication with the first fluidic port of the first microfluidic substrate and produces a fluidic path comprised of the sample-preparation column and the analytical chromatographic column; and

    a diluent source fluidically coupled to the first fluidic port, the diluent source supplying a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.

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