Analysis of dried blood spot samples in a microfluidic system with dilution of extracted samples
First Claim
1. A chromatography apparatus comprising:
- a first microfluidic substrate having a first fluidic channel comprising an analytical chromatographic column and a first fluidic port on one side of the first microfluidic substrate, the first fluidic port opening at a head end of the analytical chromatographic column; and
a second microfluidic substrate having a second fluidic channel comprising a sample-preparation column, a second fluidic port on a first side of the second microfluidic substrate and opening into an end of the sample-preparation column, the sample-preparation column terminating at the head end of the analytical column, and a third fluidic port on a second side of the second microfluidic substrate;
a dried blood spot (DBS) collection device holding one or more dried biological samples, the DBS collection device being directly coupled to the second microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the second fluidic channel of the microfluidic substrate such that the second fluidic port of the second microfluidic substrate receives an extraction of that biological sample and passes the extracted biological sample into the sample-preparation column;
wherein the second side of the second microfluidic substrate is coupled to the first microfluidic substrate such that the third fluidic port of the second microfluidic substrate is in fluidic communication with the first fluidic port of the first microfluidic substrate and produces a fluidic path comprised of the sample-preparation column and the analytical chromatographic column; and
a diluent source fluidically coupled to the first fluidic port, the diluent source supplying a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.
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Abstract
An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.
27 Citations
24 Claims
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1. A chromatography apparatus comprising:
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a first microfluidic substrate having a first fluidic channel comprising an analytical chromatographic column and a first fluidic port on one side of the first microfluidic substrate, the first fluidic port opening at a head end of the analytical chromatographic column; and a second microfluidic substrate having a second fluidic channel comprising a sample-preparation column, a second fluidic port on a first side of the second microfluidic substrate and opening into an end of the sample-preparation column, the sample-preparation column terminating at the head end of the analytical column, and a third fluidic port on a second side of the second microfluidic substrate; a dried blood spot (DBS) collection device holding one or more dried biological samples, the DBS collection device being directly coupled to the second microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the second fluidic channel of the microfluidic substrate such that the second fluidic port of the second microfluidic substrate receives an extraction of that biological sample and passes the extracted biological sample into the sample-preparation column; wherein the second side of the second microfluidic substrate is coupled to the first microfluidic substrate such that the third fluidic port of the second microfluidic substrate is in fluidic communication with the first fluidic port of the first microfluidic substrate and produces a fluidic path comprised of the sample-preparation column and the analytical chromatographic column; and a diluent source fluidically coupled to the first fluidic port, the diluent source supplying a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method of separating a biological sample into constituent components, the method comprising:
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coupling a dried blood spot (DBS) collection device, holding at least one dried biological sample, directly to a first fluidic channel on a first microfluidic substrate, the first fluidic channel configured as a sample-preparation column; extracting an analyte from the dried biological sample and passing the extracted biological sample into the first fluidic channel comprising the sample-preparation column of the microfluidic substrate; coupling the first microfluidic substrate to a second microfluidic substrate such that a first fluidic port of the first microfluidic substrate is in fluidic communication with a second fluidic port of the second microfluidic substrate and produces a fluidic path comprised of the sample-preparation column and the analytical chromatographic column; passing the extracted biological sample that elutes from the sample-preparation column into a second fluidic channel on the second microfluidic substrate, the second fluidic channel comprising an analytical chromatographic column diluting the extracted biological sample in the second fluidic channel before the extracted biological sample reaches the analytical chromatographic column; and separating, by the analytical chromatographic column, the diluted biological sample into constituent components. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification