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Compositions and methods for enhancing the biological response to chemical agents and physical stimuli

  • US 10,029,089 B2
  • Filed: 10/14/2011
  • Issued: 07/24/2018
  • Est. Priority Date: 10/15/2010
  • Status: Active Grant
First Claim
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1. A method of enhancing a cell response to a high frequency, low magnitude physical stimulus, the method comprising:

  • providing a cultured cell;

    providing a device configured to deliver the high frequency, low magnitude physical stimulus toward the cultured cell;

    exposing the cultured cell to the physical stimulus, wherein the physical stimulus is applied to the cultured cell for a first session comprising about one second to about one hour;

    providing for a refractory period wherein the same cultured cell is free of the physical stimulus for about the next 1-3 hours; and

    exposing the same cultured cell to the physical stimulus for a second session comprising about one second to about one hour immediately after the about 1-3 hours of being free of the physical stimulus,wherein the response is enhanced relative to a cultured cell receiving the same stimulus without the refractory period, and wherein the cultured cell is a purified tissue culture.

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