Optical system for chemical and/or biochemical reactions

• US 10,029,227 B2
• Filed: 01/08/2010
• Issued: 07/24/2018
• Est. Priority Date: 01/08/2009
• Status: Active Grant

• Current Assignee: IT-IS International Limited (Novacyt SAS)
• Original Assignee: IT-IS International Limited (Novacyt SAS)
• Inventors: Howell, James Richard, Webster, Benjamin Masterman, Clark, Mark Quentin, Howell, Richard Alfred
• Primary Examiner(s): Siefke, Samuel P
• Application Number:
  US13/143,720
• Publication Number:
  US 20120014835A1
• Time in Patent Office: 3,119 Days
• Field of Search: None
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 2201/0826 Fibre array at source, distributing
• CPC Class Codes: G01N 2201/0833 Fibre array at detector, resolving

Abstract

An apparatus for detecting spectra in light emanating from chemical or biochemical reactions occurring in at least one reaction vessel (3) of a plurality of reaction vessels is disclosed. Each reaction vessel (3) has a receptacle portion having an emitting area from which light can emanate. The apparatus may include a masking element (5) having an array of apertures (6) through which light from each reaction vessel (3) can escape. A plurality of light waveguides (7) are arranged to guide light from the apertures (6) in the masking element (5) to a light dispersing device (8) for dispersing the light from each waveguide (7) into a dispersed spectrum. A light detecting device (10) detects specific spectra in the dispersed spectra of light substantially simultaneously. In one embodiment, the apertures (6) are substantially smaller in size than the emitting area of the reaction vessel, but in another embodiment, the apertures (6) are substantially similar in size to the emitting area of the reaction vessel and the light waveguides (7) have a diameter that tapers from a first end substantially similar in diameter to the area of the top of the reaction vessel to a second end that is substantially smaller in diameter. In an alternative embodiment, the light waveguides are not used, when light from the small apertures is directed straight at the light dispersing device. In a further alternative embodiment, several light waveguides are provided for each reaction vessel, each waveguide directing light to a different detector for detecting a different specific spectrum.

Claims

1. Apparatus for detecting light, the apparatus comprising:

a plurality of reaction vessels, each of the reaction vessels being mounted to a heater block of a thermal cycler, each of the reaction vessels comprising a receptacle portion therein having an interior volume in which a chemical or biochemical reaction may occur and within which volume the light may be produced, each of the reaction vessels having an emitting area through which at least part of the light produced within the interior volumes of the reaction vessels passes to emanate from the reaction vessels, wherein the heater block is controlled to thermally cycle the interior volumes of the reaction vessels;

a masking element having an array of apertures arranged so that there is at least one aperture adjacent each of the emitting areas of the reaction vessels and at least a part of the light produced in the interior volumes of the receptacle portions of the reaction vessels passes therethrough, at least one of the apertures being smaller than the emitting area of the corresponding reaction vessel;

a light detecting device comprising at least one detector and controlling electronics programmed to control the at least one detector so that at least part of the light that is produced in the interior volumes of the receptacle portions of all of the reaction vessels in which light is produced is detected by the light detecting device; and

a plurality of light waveguides comprising at least one light waveguide for each reaction vessel, each of the light waveguides arranged to guide at least a part of the light from a respective aperture of the array of apertures in the masking element to the light detecting device, the light waveguides having first ends mounted to or in at least one of the respective apertures in the masking element, which respective apertures have a size similar to that of the first ends of the respective light waveguides, wherein the controlling electronics of the light detecting device are programmed to control the at least one detector so that the light received from each of the reaction vessels in which light is produced is detected simultaneously by the light detecting device.

2. Apparatus according to claim 1, further comprising a light dispersing device for dispersing the light that escapes from the small apertures in the masking element into a dispersed spectrum.

3. Apparatus according to claim 2, further comprising a plurality of light waveguides arranged to guide light from the small apertures in the masking element to the light dispersing device.

4. Apparatus according to claim 3 wherein the light detecting device comprises a plane onto which the dispersed spectra of light from the apertures is produced, and the at least one detector detects specific spectra within the dispersed spectra.

5. Apparatus according to claim 3, wherein the light dispersing device comprises a light splitting device for dispersing the light into different wavebands.

6. Apparatus according to claim 1, wherein the masking element comprises at least two of the apertures associated with each of the reaction vessels, each of the plurality of light waveguides being arranged to guide light from a respective aperture to the light detecting device, wherein one light waveguide associated with each of the reaction vessels guides the light to one portion of the light detecting device for detecting one specific spectrum of the light and another light waveguide associated with each of the reaction vessels guides the light to another portion of the light detecting device for detecting one specific spectrum of the light.

7. Apparatus according to claim 1, further comprising a further light waveguide for each of the reaction vessels arranged between a further aperture in the masking element adjacent each of the reaction vessels and an excitation light source for guiding excitation light from the excitation light source to each of the reaction vessels.

8. Apparatus according to claim 1, further comprising one or more additional light waveguides arranged to guide light from one or more excitation light sources to the light detecting device.

9. Apparatus according to claim 1, wherein the emitting area is provided in a wall of each of the reaction vessels and the masking element is formed by the heater block of the thermal cycler.

10. Apparatus according to claim 1, wherein the emitting area is provided in a bottom of each of the reaction vessels and the masking element is formed by the heater block of the thermal cycler.

11. Apparatus for detecting light, said apparatus comprising:

a plurality of reaction vessels, each of the reaction vessels being mounted to a heater block of a thermal cycler, each of the reaction vessels comprising a receptacle portion therein having an interior volume which contains a chemical or biochemical reaction of at least one reagent therein and within which interior volume the light is produced and emanates, and each reaction vessel having an emitting area through which at least part of the light produced within the interior volume of the reaction vessel thereof passes to emanate from the receptacle portion thereof, wherein the chemical or biochemical reaction of the reagent within the receptacle portion of at least a first reaction vessel of the reaction vessels may produce light that can emanate through the emitting area of the first reaction vessel, wherein the heater block is controlled to thermally cycle the interior volumes of the reaction vessels;

a masking element having an aperture adjacent each of the reaction vessels, the apertures being arranged so that at least a part of the light produced in the interior volumes of the receptacle portions of the reaction vessels passes simultaneously through the apertures;

a light dispersing device and a plurality of light waveguides arranged to guide at least part of the light from the apertures in the masking element to the light dispersing device for dispersing the light from each of the light waveguides into a dispersed spectrum, the light waveguides having first ends mounted to or in a respective aperture in the masking element, which respective apertures have a size similar to that of the first ends of the respective light waveguides; and

a light detecting device comprising at least one detector and controlling electronics programmed to control the at least one detector, the light detecting device being arranged to receive the dispersed spectra and the controlling electronics of the light detecting device being programmed to control the at least one detector so that the light detecting device simultaneously detects specific spectra of light within the dispersed spectra.

12. Apparatus according to claim 11, further comprising an output array element having a plurality of output apertures arranged in a predetermined array adjacent the light dispersing device, wherein each of the light waveguides comprises a first end constrained to receive light from a respective one of the apertures in the masking element and a second end constrained at a respective one of the apertures of the output array element to direct light to the light dispersing device.

13. Apparatus according to claim 11, wherein each of the apertures in the masking element have a size smaller than the emitting area of the receptacle portion of the reaction vessel thereof, and the light waveguides are arranged to capture at least a part of the light from the apertures in the masking element, the apparatus further comprising a further light waveguide for each of the reaction vessels arranged between a further aperture in the masking element adjacent each of the reaction vessels and an excitation light source for guiding excitation light from the excitation light source to the reagent within the receptacle portion of the first reaction vessel of the reaction vessels.

14. Apparatus according to claim 12, wherein each of the apertures in the masking element has a size similar to the emitting area of the receptacle portion of the reaction vessel thereof, and each of the light waveguides tapers in diameter from their first end thereof, which has a diameter similar to a respective one of the apertures in the masking element, and the second end of each of the light waveguides is smaller in diameter than the first end thereof.

15. Apparatus according to claim 12, wherein the light detecting device comprises a plane onto which the dispersed spectrum from the dispersing element is produced, and the at least one detector detects specific spectra within the dispersed spectra, and the array of the output apertures in the output array element is arranged so that the dispersed spectra on the plane of the light detecting device do not overlap, at least within the spectral range where there is light emitted from the reaction vessels and passed to the light detecting device.

16. Apparatus according to claim 15, wherein the array of output apertures in the output array element has a smaller area than the array of apertures in the masking element.

17. Apparatus according to claim 11, further comprising a plurality of excitation light sources, which provide excitation light of the same or different spectra, the excitation light from each of the excitation light sources being guided to each of the reaction vessels.

18. Apparatus according to claim 11, further comprising one or more additional light waveguides arranged to guide excitation light from one or more excitation light sources to the light detecting device.

19. Apparatus according to claim 11, wherein the emitting area is provided in a wall of each of the reaction vessels and the masking element is formed by the heater block of the thermal cycler.

20. Apparatus according to claim 11, wherein the emitting area is provided in a bottom of each of the reaction vessels and the masking element is formed by the heater block of the thermal cycler.

21. Apparatus for detecting spectra in light emanating from a plurality of reaction vessels of an array of reaction vessels, each of the reaction vessels being mounted to a heater block of a thermal cycler, each of the reaction vessels comprising a receptacle portion therein in which a reagent is contained and a chemical or biochemical reaction is occurring and from which light is emanating from within the receptacle portion, each of the reaction vessels having an emitting area through which the light emanating from within the receptacle portion thereof emanates from the receptacle portion thereof, wherein at least a part of the light emanating from within the receptacle portions of the reaction vessels passes through the emitting areas of the reaction vessels, wherein the heater block is controlled to thermally cycle the interior volumes of the reaction vessels, the apparatus comprising a plurality of light waveguides comprising at least one light waveguide associated with each of the reaction vessels, the light waveguides being arranged to guide at least part of the light from the emitting areas of the reaction vessels to a light dispersing device for dispersing the light from each of the light waveguides into a dispersed spectrum of light, a light detecting device comprising at least one detector and controlling electronics programmed to control the at least one detector so that the light detecting device simultaneously detects specific spectra in the dispersed spectra of light and at least one excitation arrangement for providing excitation light to the reagent within the receptacle portion of each of the reaction vessels.

22. Apparatus according to claim 21, wherein the excitation arrangement comprises an excitation light source and a second light waveguide associated with each of the reaction vessels for guiding excitation light from the excitation light source to the receptacle portion of each reaction vessel.

23. Apparatus according to claim 21, wherein the excitation arrangement comprises an excitation light source arranged in or adjacent the receptacle portion of each of the reaction vessels.

24. Apparatus according to claim 21, wherein the emitting area is provided in a wall of each of the reaction vessels and the masking element is formed by the heater block of the thermal cycler.

25. Apparatus according to claim 21, wherein the emitting area is provided in a bottom of each of the reaction vessels and the masking element is formed by the heater block of the thermal cycler.