Long-acting polypeptides and methods of producing same
First Claim
1. A polypeptide consisting of an interferon and optionally a signal peptide, wherein a first chorionic gonadotropin carboxy terminal peptide is attached to the amino terminus of said interferon, and a second and third chorionic gonadotropin carboxy terminal peptide are attached to the carboxy terminus of said interferon, wherein said third chorionic gonadotropin carboxy terminal peptide is attached in tandem to said second chorionic gonadotropin carboxy terminal peptide.
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Abstract
A polypeptide and polynucleotides encoding same comprising one carboxy-terminal peptide (CTP) of chorionic gonadotrophin attached to an amino terminus of a cytokine and two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a carboxy terminus of a cytokine are disclosed. Pharmaceutical compositions comprising the polypeptide and polynucleotides of the invention and methods of using same are also disclosed.
145 Citations
16 Claims
- 1. A polypeptide consisting of an interferon and optionally a signal peptide, wherein a first chorionic gonadotropin carboxy terminal peptide is attached to the amino terminus of said interferon, and a second and third chorionic gonadotropin carboxy terminal peptide are attached to the carboxy terminus of said interferon, wherein said third chorionic gonadotropin carboxy terminal peptide is attached in tandem to said second chorionic gonadotropin carboxy terminal peptide.
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16. A method of extending the biological half-life of an interferon, comprising attaching two or three chorionic gonadotropin carboxy terminal peptides to said interferon, wherein a first chorionic gonadotropin carboxy terminal peptide is attached to an amino terminus of said interferon, and either only a second chorionic gonadotropin carboxy terminal peptide is attached to the carboxy terminus of said interferon or two chorionic gonadotropin carboxy terminal peptides are attached to a carboxy terminus of said interferon, thereby extending the biological half-life of said interferon.
Specification