Single-walled carbon nanotube biosensor for detection of glucose, lactate, and urea
First Claim
1. A microscale multiplex biosensor configured for real time, simultaneous detection of two or more different chemical agents selected from the group consisting of D-glucose, L-lactate, and urea, the biosensor comprising:
- a substrate;
a conductive layer attached to a surface of the substrate, the conductive layer forming at least first and second pairs of electrodes with an insulating gap between the electrodes of each pair; and
a first conductive bridge between the first pair of electrodes and a second conductive bridge between the second pair of electrodes, each conductive bridge consisting essentially of one or more functionalized single-walled carbon nanotubes contacting the pair of electrodes and bridging the gap between the pair of electrodes;
wherein the one or more nanotubes of the first conductive bridge are functionalized via a linker with a first enzyme that reacts with a first chemical agent selected from the group consisting of D-glucose, L-lactate, and urea, whereby the conductivity of the first conductive bridge is modified;
wherein the one or more nanotubes of the second conductive bridge are functionalized via a linker with a second enzyme that reacts with a second chemical agent selected from the group consisting of D-glucose, L-lactate, and urea, wherein the second chemical agent is different from the first chemical agent, whereby the conductivity of the second conductive bridge is modified; and
wherein the linker is 1-pyrenebutanoic acid succinimidyl ester.
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Accused Products
Abstract
A single-walled carbon nanotube-based micron scale multiplex biosensor is provided that enables the detection of glucose, lactate, and urea. The sensor is based on modification of semiconducting single-walled carbon nanotubes using a linker that non-covalently associates with the nanotubes and covalently couples to an enzyme. Reaction of a physiological substrate with the enzyme results in increased resistance of the nanotubes within the sensor. The sensor is suitable for use in patient monitoring, particularly in a clinical setting. Incorporation of read out electronics and an RF signal generator into the sensor device enables it to communicate to a relay station or remote receiver. Methods are also provided for fabricating the biosensor device and using the device for detection.
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Citations
11 Claims
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1. A microscale multiplex biosensor configured for real time, simultaneous detection of two or more different chemical agents selected from the group consisting of D-glucose, L-lactate, and urea, the biosensor comprising:
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a substrate; a conductive layer attached to a surface of the substrate, the conductive layer forming at least first and second pairs of electrodes with an insulating gap between the electrodes of each pair; and a first conductive bridge between the first pair of electrodes and a second conductive bridge between the second pair of electrodes, each conductive bridge consisting essentially of one or more functionalized single-walled carbon nanotubes contacting the pair of electrodes and bridging the gap between the pair of electrodes; wherein the one or more nanotubes of the first conductive bridge are functionalized via a linker with a first enzyme that reacts with a first chemical agent selected from the group consisting of D-glucose, L-lactate, and urea, whereby the conductivity of the first conductive bridge is modified; wherein the one or more nanotubes of the second conductive bridge are functionalized via a linker with a second enzyme that reacts with a second chemical agent selected from the group consisting of D-glucose, L-lactate, and urea, wherein the second chemical agent is different from the first chemical agent, whereby the conductivity of the second conductive bridge is modified; and wherein the linker is 1-pyrenebutanoic acid succinimidyl ester. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification