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Single-walled carbon nanotube biosensor for detection of glucose, lactate, and urea

  • US 10,031,102 B2
  • Filed: 08/05/2014
  • Issued: 07/24/2018
  • Est. Priority Date: 08/05/2013
  • Status: Active Grant
First Claim
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1. A microscale multiplex biosensor configured for real time, simultaneous detection of two or more different chemical agents selected from the group consisting of D-glucose, L-lactate, and urea, the biosensor comprising:

  • a substrate;

    a conductive layer attached to a surface of the substrate, the conductive layer forming at least first and second pairs of electrodes with an insulating gap between the electrodes of each pair; and

    a first conductive bridge between the first pair of electrodes and a second conductive bridge between the second pair of electrodes, each conductive bridge consisting essentially of one or more functionalized single-walled carbon nanotubes contacting the pair of electrodes and bridging the gap between the pair of electrodes;

    wherein the one or more nanotubes of the first conductive bridge are functionalized via a linker with a first enzyme that reacts with a first chemical agent selected from the group consisting of D-glucose, L-lactate, and urea, whereby the conductivity of the first conductive bridge is modified;

    wherein the one or more nanotubes of the second conductive bridge are functionalized via a linker with a second enzyme that reacts with a second chemical agent selected from the group consisting of D-glucose, L-lactate, and urea, wherein the second chemical agent is different from the first chemical agent, whereby the conductivity of the second conductive bridge is modified; and

    wherein the linker is 1-pyrenebutanoic acid succinimidyl ester.

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