Biochemical analysis apparatus and rotary valve

• US 10,036,065 B2
• Filed: 01/30/2017
• Issued: 07/31/2018
• Est. Priority Date: 10/01/2010
• Status: Active Grant

• Current Assignee: Oxford Nanopore Technologies Limited
• Original Assignee: Oxford Nanopore Technologies Limited
• Inventors: Jones, Anthony
• Primary Examiner(s): Wecker, Jennifer
• Application Number:
  US15/419,121
• Publication Number:
  US 20170327880A1
• Time in Patent Office: 547 Days
• CPC Class Codes: B01L 2300/0663 Whole sensors
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: F16K 11/0743 with both the supply and the discharge passages being on one side of the closure plates
• CPC Class Codes: F16K 11/085 with cylindrical plug
• CPC Class Codes: G01N 1/26 with provision for intake from several spaces
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: G01N 33/48721 Investigating individual macromolecules, e.g. by translocation through nanopores Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68
• CPC Class Codes: G01N 33/5306 Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743
• CPC Class Codes: Y10T 137/86871 Plug

Abstract

An analysis apparatus for performing biochemical analysis of a sample using nanopores comprises: a sensor device that that supports plural nanopores, reservoirs holding material for performing the analysis; a fluidics system; and plural containers for receiving respective samples, all arranged in a cartridge that is removably attachable to an electronics unit arranged to generate drive signals to perform signal processing circuit to generate output data representing the results of the analysis. The fluidics system supplies samples selectively from the containers to the sensor device using a rotary valve. In one valve, a stator defines a plurality of first ports arranged around the rotational axis and a collection chamber extending in around the axis of rotation in communication with a second port. A rotor provides a passage extending between the collection chamber and a position in communication with any one of the plurality of first ports. In another valve a stator defining a plurality of first ports in an annular surface facing the rotational axis, and a rotor is mounted inside a liner arranged between the annular surface of the stator and a facing annular surface of the rotor. The liner has a greater compliance than the rotor and stator to facilitate sealing.

Claims

1. An analysis apparatus for performing biochemical analysis of a sample using nanopores, the analysis apparatus comprising:

a sensor device configured to support a plurality of nanopores and being configured to perform biochemical analysis of a sample using the nanopores;

at least one reservoir separate from the sensor device for holding material for performing the biochemical analysis;

a fluidics system configured to controllably supply material from the at least one reservoir to the sensor device; and

a plurality of containers configured for receiving respective samples in parallel, the fluidics system being configured to supply the samples selectively from the containers to the sensor device,

wherein the sensor device comprises a chamber for holding the material supplied from the reservoir and the samples supplied from the plurality of containers.

2. An analysis apparatus according to claim 1, wherein the analysis apparatus comprises:

a body on which the sensor device, the at least one reservoir and the fluidics system are mounted, and

a container element that is separate from the body and attachable thereto, the plurality of containers being formed in the container element.

3. An analysis apparatus according to claim 2, wherein the container element is a well plate, the containers being wells formed in the well plate.

4. An analysis apparatus according to claim 3, wherein the fluidics system comprises a plurality of nozzles arranged to protrude into the wells of the well plate when attached to the body.

5. An analysis apparatus according to claim 1, wherein the fluidics system includes a valve having an outlet port and a plurality of inlet ports corresponding to the plurality of containers and being configured to selectively connect one of the inlet ports to the outlet port, the fluidics system including channels connecting the containers to the corresponding inlet ports and channels connecting the outlet port to the sensor device.

6. An analysis apparatus according to claim 1, wherein the plurality of containers comprises 24 containers or more.

7. An analysis apparatus according to claim 1, further comprising a controller configured to measure a performance target of the biochemical analysis and control the analysis to meet the performance target.

8. An analysis apparatus according to claim 7, wherein the controller is configured to control the analysis to utilize a selection of the plurality of containers in sequence, the selection of the plurality of containers containing the same sample, until the performance target is met.

9. An analysis apparatus according to claim 1, wherein the analysis apparatus is a cartridge for cooperation with another device.

10. An analysis apparatus according to claim 1, further comprising a waste reservoir for disposal of waste products from the sensor device.

11. An analysis apparatus according to claim 10, wherein the fluidics system comprises an output pump for pumping fluids out of the sensor device through an outlet channel connected to the waste reservoir for disposal of the fluids.

12. An analysis apparatus according to claim 1, wherein the at least one reservoir holds a sufficient volume of material for performing the biochemical analysis multiple times by repeatedly refreshing the sensor device with material from the at least one reservoir.