Method for optical detection of bio-contaminants

• US 10,036,705 B2
• Filed: 04/29/2016
• Issued: 07/31/2018
• Est. Priority Date: 02/26/2013
• Status: Active Grant

• Current Assignee: National Optics Institute, Steris Corporation
• Original Assignee: National Optics Institute, Steris Corporation
• Inventors: Rochette, Daniel, Gallant, Pascal, Mermut, Ozge, Baribeau, Francois, Noiseux, Isabelle, Gosselin, Isabelle
• Primary Examiner(s): Xu, Xiaoyun R
• Application Number:
  US15/142,309
• Publication Number:
  US 20160245752A1
• Time in Patent Office: 823 Days
• Field of Search: None
• CPC Class Codes: A61L 2/28 Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour apparatus involving enzymes or microorganisms C12M1/34; methods involving enzymes or microorganisms C12Q1/00
• CPC Class Codes: A61L 2202/24 Medical instruments, e.g. endoscopes, catheters, sharps
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/94 Investigating contamination, e.g. dust G01N21/85 takes precedence
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 2201/0697 Pulsed lasers
• CPC Class Codes: G01N 2201/10 Scanning
• CPC Class Codes: G01N 2201/105 Purely optical scan

Abstract

A method for optical detection of residual soil on articles (such as medical instruments and equipment), after completion of a washing or a rinsing operation by a washer. A soil detection system provides an indication of soil on the articles by detecting luminescent radiation emanating from the soil in the presence of ambient light.

Claims

1. A method for detecting presence of soil on an article, said method comprising:

introducing a luminescent agent to a detergent during a wash cycle of a washing apparatus, wherein the luminescent agent is a fluorescent agent that emits fluorescent light at a fluorescence wavelength, said luminescent agent bound to soil present on the article;

rinsing the article to remove unbound luminescent agent;

exposing the article to laser light blinking at a modulation frequency wherein the modulation frequency is lower than a blinking frequency of an ambient lighting source;

filtering the light emanating from said article to pass light at the fluorescence wavelength, said light emanating from said article including reflected ambient light reflected by the article and fluorescent light blinking at the modulation frequency emitted by exciting the luminescent agent bound to the soil with the laser light;

detecting the filtered light emanating from said article by a light detector and generating light data indicative of the reflected ambient light and the blinking fluorescent light; and

determining the presence of soil on the article based upon the light data generated from the filtered light received by the light detector, wherein said step of determining the presence of soil includes;

determining from the light data detected fluorescent light features at the fluorescence wavelength that are blinking at the modulation frequency and thus indicative of soil,

determining from the light data detected features at the fluorescence wavelength that are non-blinking at the modulation frequency and thus identified as ambient light reflections, and

identifying from the light data the respective detected blinking and non-blinking features, thereby discriminating between soil fluorescence and ambient light reflections.

2. A method according to claim 1, wherein said modulation frequency is around 10 Hz.

3. A method according to claim 1, wherein said fluorescent agent is bio-compatible.

4. A method according to claim 1, wherein said fluorescent agent is fluorescein.

5. A method according to claim 4, wherein said fluorescein concentration is in the range of about 0.001 mM to 90 mM.

6. A method according to claim 1, wherein said soil is an organic residue including one or more of the following:

blood, fat, mucous, lipids, carbohydrates, bone, hair, protein, and food product.

7. A method according to claim 1, wherein said luminescent agent can withstand temperatures exceeding 80°

C.

8. A method for detecting presence of soil on an article, said method comprising:

providing the article with a fluorescent agent bound to the soil present on the article, wherein, upon incidence of excitation light, the fluorescent agent emits fluorescent light at a fluorescence wavelength;

exposing the article to the excitation light blinking at a modulation frequency wherein the modulation frequency is lower than a blinking frequency of an ambient lighting source;

detecting light emanating from said article at the fluorescence wavelength, said light emanating from said article including reflected ambient light reflected by the article and blinking fluorescent light emitted by excitation of the luminescent agent bound to the soil by the excitation light that is blinking at the modulation frequency;

determining presence of soil on the article based upon the detected light emanating from the article, said step of determining the presence of soil includes;

detecting blinking features on the article corresponding to the fluorescent light at the modulation frequency and thus indicative of soil,

detecting features at the fluorescence wavelength that are non-blinking at the modulation frequency and thus identified as ambient light reflections, and

wherein said step of determining the presence of soil further includes identifying the detected blinking and non-blinking features and thereby discriminating between soil fluorescence and ambient light reflections.

9. A method according to claim 8, wherein said step of exposing the article to excitation light comprises exposing the article to laser light blinking at the modulation frequency.

10. A method according to claim 8, wherein said step of exposing the article to excitation light comprises scanning over surfaces of the article while activating the excitation light.

11. A method according to claim 10, wherein said step of scanning comprises manually moving a handheld scanning unit over the surfaces of the article by a user.

12. A method according to claim 10, wherein said step of scanning comprises automatically activating at least one scanning unit for scanning at least one surface of the article within a chamber.

13. A method according to claim 8, wherein, prior to said step of detecting, filtering the light emanating from said article at predetermined frequencies to pass the fluorescent light at the fluorescence wavelength.

14. A method according to claim 13, wherein said fluorescence wavelength is green light at about 513 nm and wherein the step of filtering comprises passing both the reflected ambient light and the emitted fluorescent light of the soil through a filter which filters out all but green light.

15. A method according to claim 13, wherein said step of detecting comprises receiving filtered light passed at the fluorescence wavelength by a light detector.

16. A method according to claim 8, wherein said step of providing the article with a fluorescent agent comprises introducing the fluorescent agent to a detergent during a wash cycle of a washing apparatus.

17. A method according to claim 16, wherein said step of introducing the fluorescent agent is followed by rinsing the article to remove unbound fluorescent agent during the wash cycle.

18. A method according to claim 8, further comprising displaying the detected blinking features indicative of soil on a display unit, wherein non-blinking features or features blinking at frequencies other than the modulation frequency identified as ambient light reflections are not displayed on the display unit.

19. A method for detecting presence of soil on an article, said method comprising:

introducing a luminescent agent to a detergent during a wash cycle of a washing apparatus, wherein the luminescent agent is a fluorescent agent that emits fluorescent light at a fluorescence wavelength, said luminescent agent bound to soil present on the article;

rinsing the article to remove unbound luminescent agent;

scanning over surfaces of the article with a handheld scanning unit comprising laser light blinking at a modulation frequency wherein the modulation frequency is lower than a blinking frequency of an ambient lighting source;

filtering light emanating from said article to pass light at the fluorescence wavelength, said light emanating from said article including reflected ambient light reflected by the article and blinking fluorescent light emitted by exciting the luminescent agent bound to the soil with the laser light blinking at the modulation frequency;

detecting the filtered light emanating from said article and generating light data corresponding to the reflected ambient light and the blinking fluorescent light;

determining the presence of soil on the article based upon the filtered light, wherein said step of determining the presence of soil includes determining from the light data blinking fluorescent light features blinking at the modulation frequency identified as soil, and non-blinking features identified as ambient light reflections, and thereby discriminating between soil fluorescence and ambient light reflections; and

displaying detected blinking features indicative of soil on a display unit, wherein non-blinking features or features blinking at frequencies other than the modulation frequency identified as ambient light reflections are not displayed.