Device and method for transferring a target between locations

• US 10,040,062 B2
• Filed: 01/14/2014
• Issued: 08/07/2018
• Est. Priority Date: 01/14/2014
• Status: Active Grant

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Beebe, David J., Casavant, Ben P., Guckenberger, David J., Berry, Scott M.
• Primary Examiner(s): Warden, Jill A
• Assistant Examiner(s): Brazin, Jacqueline
• Application Number:
  US14/154,695
• Publication Number:
  US 20150196907A1
• Time in Patent Office: 1,666 Days
• Field of Search: 422538, 436526
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0657 Pipetting powder
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2400/021 non contact spotting by inertia, i.e. abrupt deceleration of the nozzle or pin
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: B01L 3/5088 confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: B01L 3/563 Joints or fittings in general F16L; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors

Abstract

A device and method are proved for transferring a target from a first location to a second location. The target is bound to solid phase substrate to form a target bound solid phase substrate. The device includes transfer surface for receiving the target bound solid phase substrate thereon for transfer. The transfer surface movable between a first position wherein the transfer surface is aligned with the first location and spaced therefrom by a distance and a second position wherein the transfer surface is aligned with the second location. An alignment structure aligns the transfer surface with respect to the second location, with the transfer surface in the second position. A force is movable between an attraction position wherein the target bound solid phase substrate are drawn toward the transfer surface and a discharge position wherein the target bound solid phase substrate are free of the force.

Claims

1. A device for transferring a target received in a first fluid at a first location to a second location, the target being bound to a solid phase substrate to form a target bound solid phase substrate, comprising:

a transfer structure having a first surface and a second surface, the second surface defining a transfer surface including a first region for receiving the target bound solid phase substrate thereon for transfer and being movable between a first position wherein the transfer surface is axially aligned with an apex of the first fluid at the first location and spaced from the apex of the first fluid by a non-liquid air gap and a second position wherein the transfer surface is aligned with the second location which is spaced from the first location;

an alignment structure for aligning the transfer surface with respect to the second location, with the transfer surface in the second position; and

a force generator adjacent the first surface of the transfer structure and being configured to generate a force, the force generator being movable with respect to the transfer surface between a non-attraction position wherein the target bound solid phase substrate is free of the force and an attraction position;

wherein, with the transfer surface in the first position and the force generator in the attraction position;

wherein;

the transfer surface is spaced from the first location by a distance such that the force is sufficient to attract the target bound solid phase substrate; and

the target bound solid phase substrate received in the first fluid at the first location exits the first fluid and travels through the non-liquid air gap toward the first region of the transfer surface in response to the attraction of the target bound solid phase substrate to the force.

2. The device of claim 1 further comprising a sample container at the first location for receiving the first fluid and having an inner surface, the inner surface of the sample container being hydrophobic and causing the first fluid in the sample container to have a non-concave meniscus.

3. The device of claim 1 further comprising:

a sample container at the first location for receiving the first fluid and having an inner surface; and

an insert receiveable in the sample container along the inner surface thereof, the insert causing the first fluid in the sample container to have a non-concave meniscus.

4. The device of claim 1 further comprising a second fluid received in a receptacle at the second location, the receptacle having an inner surface and an upper edge.

5. The device of claim 4 wherein the alignment structure includes a plate, the transfer surface extending along a first side of the plate and overlapping the upper edge of the receptacle with the transfer surface in the second position.

10. The device of claim 5 wherein the plate includes an upper surface on a second side thereof, the force is adjacent the upper surface of the plate with the force in the attraction position.

11. The device of claim 4 wherein the alignment structure includes a plate and a transfer element depending from a first side thereof, the transfer element terminating at the transfer surface and having an outer surface engageable with the inner surface of the receptacle with the transfer surface in the second position.

12. The device of claim 11 wherein the first side of the plate is engageable with the upper edge of the receptacle with the transfer surface in the second position.

13. The device of claim 1 wherein the force is a magnetic force.

14. The device of claim 1 further comprising an intermediate fluid disposed between the transfer surface and the first location, the target bound solid phase substrate passing through the intermediate fluid as the target bound solid phase substrate is drawn from the first location to the first region of the transfer surface.

6. A device for transferring a target received in a first fluid at a first location to a second location, the target being bound to a solid phase substrate to form a target bound solid phase substrate, comprising:

a transfer structure having a first surface and a transfer surface, the transfer surface including a first region for receiving the target bound solid phase substrate thereon for transfer and being movable between a first position wherein the transfer surface is axially aligned with an apex of the first fluid at the first location and spaced from the apex of the first fluid by a non-liquid air gap and a second position wherein the transfer surface is aligned with the second location which is spaced from the first location;

an alignment structure for aligning the transfer surface with respect to the second location, with the transfer surface in the second position; and

a force generator adjacent to the first surface of the transfer structure and being configured to generate a force, the force generator being movable with respect to the transfer surface between a non-attraction position wherein the target bound solid phase substrate is free of the force and an attraction position wherein, with the transfer surface in the first position, the force attracts the target bound solid phase substrate received in the first fluid at the first location exits the first fluid and travels through the non-liquid air gap toward the first region of the transfer surface;

wherein;

the alignment structure includes a plate and a wall depending from the transfer surface, the wall having an inner surface defining the first region of the transfer surface and an outer surface engageable with the inner surface of the receptacle with the transfer surface in the second position; and

the transfer surface extends along a first side of the plate and overlaps the upper edge of the receptacle with the transfer surface in the second position.

7. The device of claim 6 wherein:

the wall is a first wall;

the receptacle includes an outer surface; and

the alignment structure further includes a second wall depending from the transfer surface, the second wall having an inner surface engageable with the outer surface of the receptacle with the transfer surface in the second position and an outer surface.

8. A device for transferring a target received in a first fluid at a first location to a second location, the target being bound to a solid phase substrate to form a target bound solid phase substrate, comprising:

a transfer structure having a first surface and a transfer surface, the transfer surface including a first region for receiving the target bound solid phase substrate thereon for transfer and being movable between a first position wherein the transfer surface is axially aligned with an apex of the first fluid at the first location and spaced from the apex of the first fluid by a non-liquid air gap and a second position wherein the transfer surface is aligned with the second location which is spaced from the first location;

an alignment structure for aligning the transfer surface with respect to the second location, with the transfer surface in the second position;

a force generator adjacent to the first surface of the transfer structure and being configured to generate a force, the force generator being movable with respect to the transfer surface between a non-attraction position wherein the target bound solid phase substrate is free of the force and an attraction position wherein, with the transfer surface in the first position, the force attract the target bound solid phase substrate such that the target bound solid phase substrate received in the first fluid at the first location exits the first fluid and travels through the non-liquid air gap toward the first region of the transfer surface;

a second fluid received in a receptacle at the second location, the receptacle having an inner surface and an upper edge; and

a transfer fluid provided at the first region of the transfer surface; and

wherein;

the receptacle includes an outer surface; and

the alignment structure includes a wall depending from the transfer surface, the wall having an inner wall surface engageable with the outer surface of the receptacle with the transfer surface in the second position and an outer wall surface.

9. The device of claim 8 wherein the first region of the transfer surface includes a pinning element extending about the outer periphery thereof, the pinning element retaining the transfer fluid in the first region of the transfer surface.

15. A device for transferring a target from a first fluid at a first location to a second location, the target being bound to a solid phase substrate to form target bound solid phase substrate, comprising:

means for forming a non-concave meniscus with the first fluid;

a transfer structure having a first surface and a second surface,

a transfer region on the second surface of the transfer structure for receiving the target bound solid phase substrate for transfer, the transfer region movable with respect to the first fluid between a first position wherein the transfer region is axially aligned with and separated from an apex of the non-concave meniscus of the first fluid by a non-liquid air gap and a second position wherein the transfer region is aligned with the second location which is spaced from the first location;

an alignment structure for aligning the transfer region with respect to the receptacle with the transfer region in the second position;

force generator adjacent to the first surface of the transfer structure and being configured to generate a force and being movable with respect to the transfer region between a non-attraction position wherein the target bound solid phase substrate is free of the force and an attraction position;

wherein, with the transfer region in the first position and the force generator in the attraction position;

the transfer surface is spaced from the first location by a distance such that the force is sufficient to attract the target bound solid phase substrate in the first fluid; and

the target bound solid phase substrate exits the first fluid and travels through the non-liquid air gap toward the transfer region in response to the attraction of the target bound solid phase substrate to the force.

16. The device of claim 15 wherein the first fluid is received in a sample container and wherein the forming means includes an inner surface of the sample container being hydrophobic so as to cause the first fluid in the sample container to have the non-concave meniscus.

17. The device of claim 15 wherein the first fluid is received in a sample container having an inner surface, and wherein the forming means includes an insert receiveable in the sample container along the inner surface thereof, the insert causing the first fluid in the sample container to have a non-concave meniscus.

18. The device of claim 15 further comprising an intermediate fluid disposed between the transfer region and the first fluid, the target bound solid phase substrate passing through the intermediate fluid as the target bound solid phase substrate is drawn from the first fluid to the transfer region.

19. The device of claim 15 wherein the alignment structure includes a plate and the second location includes a receptacle having an inner surface and an upper edge, the transfer region extending along a first side of the plate and the plate overlaps the upper edge of the receptacle with the transfer region in the second position.

20. The device of claim 19 wherein the alignment structure further includes a wall depending from the transfer surface, the wall engageable with the receptacle with the transfer region in the second position.

21. The device of claim 15 further comprising a transfer fluid provided at the transfer region and a pinning element extending about the outer periphery of the transfer region, the pinning element retaining the transfer fluid in the transfer region.