Immortalization of epithelial cells and methods of use
First Claim
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1. A method for continuously culturing non-keratinocyte epithelial cells, comprising:
- (a) culturing non-keratinocyte epithelial (NKE) cells in the presence of feeder cells or a medium conditioned with feeder cells; and
a calcium-containing medium; and
(b) inhibiting the activity of Rho kinase (ROCK) in the feeder cells, the population of the NKE cells or both during the culturing, thereby establishing a cell culture comprising an expanded population of the NKE cells, wherein;
the cell culture does not include embryonic stem cells, andthe population of the NKE cells in the cell culture is conditionally immortalized, has an average telomere length of about 4 kb or less, and undergoes cell division.
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Abstract
The present invention is directed towards methods of culturing non-keratinocyte epithelial cells, with the methods comprising culturing non-keratinocyte epithelial cells in the presence of feeder cells and a calcium-containing medium while inhibiting the activity of Rho kinase (ROCK) in the feeder cell, the non-keratinocyte epithelial cells or both during culturing.
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16 Claims
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1. A method for continuously culturing non-keratinocyte epithelial cells, comprising:
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(a) culturing non-keratinocyte epithelial (NKE) cells in the presence of feeder cells or a medium conditioned with feeder cells; and
a calcium-containing medium; and(b) inhibiting the activity of Rho kinase (ROCK) in the feeder cells, the population of the NKE cells or both during the culturing, thereby establishing a cell culture comprising an expanded population of the NKE cells, wherein; the cell culture does not include embryonic stem cells, and the population of the NKE cells in the cell culture is conditionally immortalized, has an average telomere length of about 4 kb or less, and undergoes cell division. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification