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Methods and apparatus for synthesizing nucleic acids

  • US 10,041,110 B2
  • Filed: 07/28/2014
  • Issued: 08/07/2018
  • Est. Priority Date: 04/02/2013
  • Status: Active Grant
First Claim
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1. A method for synthesizing an oligonucleotide, comprising:

  • exposing a nucleic acid attached to a solid support to a nucleotide analog in the presence of a nucleotidyl transferase enzyme and in the absence of a nucleic acid template, thereby creating an oligonucleotide analog,wherein the nucleotide analog comprises an unmodified 3′

    hydroxyl and a cleavable terminating group comprising a charged moiety,wherein the oligonucleotide analog is not a substrate for said nucleotidyl transferase enzyme when the cleavable terminating group is attached, but the oligonucleotide analog is a substrate for said nucleotidyl transferase enzyme when the terminating group is not attached.

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