3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
First Claim
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1. A method comprising:
- exposing a compound, a biological component, or a non-biological component to a light source to remove a photocleavable moiety of the formula;
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Abstract
The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.
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Citations
26 Claims
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1. A method comprising:
exposing a compound, a biological component, or a non-biological component to a light source to remove a photocleavable moiety of the formula; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- 21. A compound of the formula:
Specification