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Primers and methods for the detection and discrimination of nucleic acids

  • US 10,041,117 B2
  • Filed: 07/11/2014
  • Issued: 08/07/2018
  • Est. Priority Date: 10/23/2001
  • Status: Active Grant
First Claim
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1. A composition comprising one or more target nucleic acid molecules and at least two different oligonucleotides, wherein said oligonucleotides are extendable by nucleic acid synthesis and wherein said oligonucleotides, when extended by nucleic acid synthesis, are incorporated into a double-stranded nucleic acid product on opposite strands, wherein said first oligonucleotide comprises a fluorescent moiety at or near its 3′

  • -terminal nucleotide and said second oligonucleotide comprises a quenching moiety at or near its 3′

    -terminal nucleotide; and

    wherein said first oligonucleotide exhibits a detectable change upon incorporation into said double-stranded nucleic acid product.

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