Bioproduction of chemicals

• US 10,047,383 B2
• Filed: 03/17/2014
• Issued: 08/14/2018
• Est. Priority Date: 03/15/2013
• Status: Active Grant

• Current Assignee: Cargill Incorporated
• Original Assignee: Cargill Incorporated
• Inventors: Liao, Hans, Mercogliano, Christopher Patrick, Wolter, Travis Robert, Louie, Michael Tai Man, Ribble, Wendy Kathleen, Lipscomb, Tanya, Spindler, Eileen Colie, Lynch, Michael
• Primary Examiner(s): Carlson, Karen Cochrane
• Application Number:
  US14/769,413
• Publication Number:
  US 20160138055A1
• Time in Patent Office: 1,611 Days
• CPC Class Codes: C07C 59/01 Saturated compounds having only one carboxyl group and containing hydroxy or O-metal groups
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12N 15/62 DNA sequences coding for fusion proteins
• CPC Class Codes: C12N 15/70 Vectors or expression systems specially adapted for E. coli
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12N 9/0008 acting on the aldehyde or oxo group of donors (1.2)
• CPC Class Codes: C12N 9/0016 with NAD or NADP as acceptor (1.4.1)
• CPC Class Codes: C12N 9/93 Ligases (6)
• CPC Class Codes: C12P 7/42 Hydroxy-carboxylic acids
• CPC Class Codes: C12P 7/52 Propionic acid; Butyric acids
• CPC Class Codes: C12Y 604/01002 Acetyl-CoA carboxylase (6.4.1.2)
• CPC Class Codes: Y02P 20/52 using catalysts, e.g. selective catalysts

Abstract

The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.

Claims

1. A genetically modified E. coli capable of producing a chemical product of interest, wherein the genetic modification includes introduction of heterologous nucleic acid sequences coding for:

an acetyl-CoA carboxylase; and

one or more polypeptides selected from the group consisting of BicA from Synechococcus species, ychM gene product of E. coli, and yidE gene product of E. coli to facilitate importation of bicarbonate from an extracellular media to within the E. coli.

2. A method of producing 3-hydroxypropionic acid (3-HP), the method comprising:

culturing the genetically modified E. coli of claim 1, wherein the genetically modified E. coli produces 3-HP.