Apparatus for optical detection of bio-contaminants

• US 10,048,203 B2
• Filed: 08/09/2016
• Issued: 08/14/2018
• Est. Priority Date: 02/26/2013
• Status: Active Grant

• Current Assignee: National Optics Institute, Steris Inc (Steris Corporation)
• Original Assignee: National Optics Institute, Steris Inc (Steris Corporation)
• Inventors: Rochette, Daniel, Gallant, Pascal, Mermut, Ozge, Baribeau, Francois, Noiseux, Isabelle, Gosselin, Isabelle
• Primary Examiner(s): Xu, Xiaoyun R
• Application Number:
  US15/232,091
• Publication Number:
  US 20160349179A1
• Time in Patent Office: 735 Days
• Field of Search: None
• CPC Class Codes: A61L 2/28 Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour apparatus involving enzymes or microorganisms C12M1/34; methods involving enzymes or microorganisms C12Q1/00
• CPC Class Codes: A61L 2202/24 Medical instruments, e.g. endoscopes, catheters, sharps
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/94 Investigating contamination, e.g. dust G01N21/85 takes precedence
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 2201/0697 Pulsed lasers
• CPC Class Codes: G01N 2201/10 Scanning
• CPC Class Codes: G01N 2201/105 Purely optical scan

Abstract

A method for optical detection of residual soil on articles (such as medical instruments and equipment), after completion of a washing or a rinsing operation by a washer. A soil detection system provides an indication of soil on the articles by detecting luminescent radiation emanating from the soil in the presence of ambient light.

Claims

1. A soil detection system for detecting presence of soil on an article, the soil detection system comprising:

a scanning unit for scanning the article with a fluorescent agent bound to soil present on the article, the fluorescent agent emitting fluorescent light at a fluorescence wavelength, wherein the scanning unit is moved across a surface of the article, said scanning unit including;

a light source for producing a laser light blinking at a modulation frequency to be incident on the article, wherein the modulation frequency is lower than a blinking frequency of an ambient lighting source,

a light filter for filtering light emanating from said article at the fluorescence wavelength, said light emanating from said article including ambient light from the ambient lighting source reflected by the article and light emitted by exciting the fluorescent agent bound to the soil with the laser light, and

a light detector for detecting the filtered light emanating from said article and generating light data corresponding thereto; and

a control unit for receiving the light data generated by the light detector to determine the presence of soil on the article based upon detection of blinking features at the fluorescence wavelength determined to be blinking at the modulation frequency and indicative of soil, compared to detected features at the fluorescence wavelength that are non-blinking at the modulation frequency and identified as ambient light reflections, thereby discriminating between soil fluorescence and ambient light reflections.

2. A soil detection system according to claim 1, further comprising a power modulator to produce a pulsed waveform that causes the laser light produced by the light source to be continuously pulsed ON and OFF at the modulation frequency.

3. A soil detection system according to claim 1, wherein the fluorescence wavelength is green light, and wherein said light filter permits only green light to pass therethrough and be received by said light detector.

4. A soil detection system according to claim 1, wherein the fluorescent agent is fluorescein that binds to soil and fluoresces under the laser light at a fluorescence wavelength of about 513 nm.

5. A soil detection system according to claim 1, wherein the laser light is at a wavelength of about 490 nm, an excitation wavelength upon which the fluorescent agent emits the fluorescent light.

6. A soil detection system according to claim 1, wherein said scanning unit includes a dichroic beamsplitter that reflects the laser light produced by the light source and passes reflected ambient light and emitted fluorescent light.

7. A soil detection system according to claim 1, wherein said scanning unit comprises a handheld scanning unit.

8. A soil detection system according to claim 7, wherein said handheld scanning unit comprises a handle grip grabbed by a user to manually move the handheld scanning unit across the surface of the article, and a trigger switch to activate the light source of the handheld scanning unit.

9. A soil detection system according to claim 1, wherein the scanning unit comprises a plurality of scanning units located within a chamber for exposing a plurality of surfaces of an article to the laser light.

10. A soil detection system according to claim 9, wherein the plurality of scanning units are automatically activated by the control unit.

11. A soil detection system according to claim 9, further comprising a tray for receiving the article, wherein the tray is moveable within the chamber with respect to the plurality of scanning units.

12. A soil detection system according to claim 9, wherein the plurality of scanning units are moveable to provide a range of motion with respect to the article.

13. A soil detection system according to claim 1, wherein the modulation frequency of the light source is about 10 Hz.

14. A soil detection system according to claim 1, wherein the blinking frequency (f) of the ambient lighting source is selected from at least one of:

f=20-60 kHz for an electronic ballast fluorescent tube light source;

f=120 Hz for an incandescent light bulb and magnetic ballast fluorescent tube light sources;

or f=240 Hz for a computer monitor.

15. A soil detection system according to claim 1, wherein the light detector operates in a continuous video mode at a frame rate that is higher than the modulation frequency.

16. A soil detection system according to claim 2, wherein the power modulator comprises a square wave modulation circuit that causes the light source to implement amplitude modulation of the laser light at the modulation frequency.

17. A soil detection system for detecting presence of soil on an article, the soil detection system comprising:

a plurality of scanning units located within a chamber for scanning a plurality of surfaces of the article having a fluorescent agent bound to soil present on the article, the fluorescent agent emitting fluorescent light at a fluorescence wavelength, each of said plurality of scanning units including;

a light source for producing a laser light blinking at a modulation frequency to be incident on the article, wherein the modulation frequency is lower than a blinking frequency of an ambient lighting source,

a light filter for filtering light emanating from said article at the fluorescence wavelength, said light emanating from said article including ambient light from the ambient lighting source reflected by the article and light emitted by exciting the fluorescent agent bound to the soil with the laser light, and

a light detector for detecting the filtered light emanating from said article and generating light data corresponding thereto; and

a control unit for receiving the light data generated by each light detector of the plurality of scanning units to determine the presence of soil on the article based upon detection of blinking features at the fluorescence wavelength determined to be blinking at the modulation frequency and indicative of soil, compared to detected features that are non-blinking at the modulation frequency and identified as ambient light reflections, thereby discriminating between soil fluorescence and ambient light reflections.