Oscillating immunoassay method and device
First Claim
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1. A method of performing a sandwich immunoassay for an analyte, said method comprising:
- mixing magnetically susceptible beads with a sample containing an analyte, wherein the beads are coated with a first antibody to the analyte and a signaling moiety;
oscillating the beads across the surface of a sensing electrode in an immunosensor coated with a second antibody to the analyte, thereby forming an antibody sandwich and immobilizing the beads on the immunosensor,wherein the sensing electrode is position in a conduit for receiving the sample containing the analyte mixed with the beads,wherein the conduit comprises two magnetic zones, andwherein the beads are oscillated in the direction of a capture motion of the beads;
washing unbound sample and beads from the immunosensor; and
detecting the signaling moiety immobilized on said immunosensor.
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Abstract
The present invention provides apparatus and methods for the rapid determination of analytes in liquid samples by immunoassays incorporating magnetic capture of beads on a sensor capable of being used in the point-of-care diagnostic field.
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Citations
23 Claims
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1. A method of performing a sandwich immunoassay for an analyte, said method comprising:
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mixing magnetically susceptible beads with a sample containing an analyte, wherein the beads are coated with a first antibody to the analyte and a signaling moiety; oscillating the beads across the surface of a sensing electrode in an immunosensor coated with a second antibody to the analyte, thereby forming an antibody sandwich and immobilizing the beads on the immunosensor, wherein the sensing electrode is position in a conduit for receiving the sample containing the analyte mixed with the beads, wherein the conduit comprises two magnetic zones, and wherein the beads are oscillated in the direction of a capture motion of the beads; washing unbound sample and beads from the immunosensor; and detecting the signaling moiety immobilized on said immunosensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification