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Cartridge device with segmented fluidics for assaying coagulation in fluid samples

  • US 10,048,281 B2
  • Filed: 09/25/2015
  • Issued: 08/14/2018
  • Est. Priority Date: 09/26/2014
  • Status: Active Grant
First Claim
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1. A sample analysis cartridge comprising:

  • an inlet chamber configured to receive a biological sample;

    a conduit fluidically connected to the inlet chamber and configured to receive the biological sample from the inlet chamber, the conduit splitting at a first junction into a first conduit and a second conduit configured to split the biological sample into at least first and second segments;

    the first conduit fluidically connected to the conduit at the first junction and configured to receive the first segment of the biological sample, the first conduit comprising a first reagent, a first sensor region comprising a first sensor, and a first fluidic lock valve downstream of the first sensor region;

    the second conduit fluidically connected to the conduit at the first junction and configured to receive the second segment of the biological sample, the second conduit comprising a second sensor region comprising a second sensor,a first flow restrictor downstream of the first junction and upstream of the second sensor region and a second reagent; and

    an air bladder connected to the inlet chamber and forming a part of a pump configured during operation;

    to displace fluids within conduits of the sample analysis cartridge;

    to independently mix the first segment of the biological sample in the first conduit to dissolve the first reagent into the first segment of the biological sample and mix the second segment of the biological sample in the second conduit to dissolve the second reagent into the second segment of the biological sample; and

    to independently position the first segment of the biological sample over the first sensor region and position the second segment of the biological sample over the second sensor region;

    wherein the first sensor region comprises at least one prothrombin time sensor, and the second sensor region comprises at least one activated partial thromboplastin time sensor;

    wherein the first reagent comprises a first thrombin-cleavable peptide with a detectable moiety, and the second reagent comprises a second thrombin-cleavable peptide with a detectable moiety; and

    wherein the first reagent is different from the second reagent.

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