Multi-spectral laser imaging (MSLI) methods and systems for blood flow and perfusion imaging and quantification
First Claim
1. A multispectral imaging system, the system comprising:
- a first light source, the first light source being one of coherent, non-coherent and partially coherent, the first light source having a first wavelength configured to produce a non-coherent illumination to image a sample;
a second coherent light source, different from the first light source, having a second wavelength, different from the first wavelength, configured to image the sample simultaneously with the first light source;
a camera configured to simultaneously receive information related to the first and second light sources from the sample, wherein light at the first wavelength is configured to image a surface of the sample into the camera and light at the second wavelength is configured to penetrate the sample and provide information related to the penetrated sample to the camera; and
a processor configured to combine the received information related to the first and second light sources and generate a synthesized image of the sample comprising surface anatomical structure and sub-surface physiology of blood flow and perfusion of the sample in terms of a blood flow rate distribution.
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Abstract
Some embodiments of the present inventive concept provide a system that uses two wavelengths of differential transmittance through a sample to apply laser speckle or laser Doppler imaging. A first of the two wavelengths is within the visible range that has zero or very shallow penetration. This wavelength captures the anatomical structure of tissue/organ surface and serves as a position marker of the sample but not the subsurface movement of blood flow and perfusion. A second wavelength is in the near Infra-Red (NIR) range, which has much deeper penetration. This wavelength reveals the underlying blood flow physiology and correlates both to the motion of the sample and also the movement of blood flow and perfusion. Thus, true motion of blood flow and perfusion can be derived from the NIR imaging measurement without being affected by the motion artifact of the target.
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Citations
23 Claims
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1. A multispectral imaging system, the system comprising:
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a first light source, the first light source being one of coherent, non-coherent and partially coherent, the first light source having a first wavelength configured to produce a non-coherent illumination to image a sample; a second coherent light source, different from the first light source, having a second wavelength, different from the first wavelength, configured to image the sample simultaneously with the first light source; a camera configured to simultaneously receive information related to the first and second light sources from the sample, wherein light at the first wavelength is configured to image a surface of the sample into the camera and light at the second wavelength is configured to penetrate the sample and provide information related to the penetrated sample to the camera; and a processor configured to combine the received information related to the first and second light sources and generate a synthesized image of the sample comprising surface anatomical structure and sub-surface physiology of blood flow and perfusion of the sample in terms of a blood flow rate distribution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for multispectral imaging in a multispectral imaging system, the method comprising:
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simultaneously imaging a sample using a first light source having a first wavelength configured to produce a non-coherent illumination and a second coherent light source, different from the first light source, having a second wavelength, different from the first wavelength; receiving information related to the first and second light sources simultaneously from the sample at a camera, wherein light at the first wavelength is configured to reflect off a surface of the sample into the camera and light at the second wavelength is configured to penetrate the sample and provide information related to the penetrated sample to the camera; and combining the received information related to the first and second light sources to generate a synthesized image of the sample comprising surface anatomical structure and sub-surface physiology of blood flow and perfusion of the sample in terms of a blood flow rate distribution, wherein at least one of the imaging, receiving and combining are performed by at least one processor. - View Dependent Claims (22)
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23. A computer program product for multispectral imaging in a multispectral imaging system, the computer program product comprising:
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a non-transitory computer readable storage medium having computer readable program code embodied in the medium, the computer readable program code comprising; computer readable program code to direct a first light source to transmit light onto a sample, the first light source having a first wavelength configured to produce a non-coherent illumination and direct a second light source to transmit light into the sample, the second light source being different from the first light source and having a second wavelength configured to produce coherent illumination, different from the first wavelength, wherein light at the first wavelength is configured to reflect off a surface of the sample into a camera and provide information regarding the sample to the camera and light at the second wavelength is configured to penetrate the sample and provide information related to the penetrated sample to the camera simultaneously with the information from the first wavelength; and computer readable program code to combine the information related to the first and second light sources received simultaneously by the camera to generate a synthesized image of the sample comprising surface anatomical structure and physiology of sub-surface blood flow and perfusion of the sample in terms of blood flow rate distribution.
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Specification