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Bioartifical lung

  • US 10,059,913 B2
  • Filed: 04/13/2015
  • Issued: 08/28/2018
  • Est. Priority Date: 06/04/2009
  • Status: Active Grant
First Claim
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1. An airway organ bioreactor apparatus, comprising:

  • an organ chamber configured to hold an organ matrix scaffold onto which a cell media is perfused to grow an organ;

    a flow perfusion system configured for perfusion seeding, the flow perfusion system comprising;

    a reservoir system configured to contain a first cell media having at least one of differentiated or regenerative cells suspended in the cell media and a second cell media,a pulmonary arterial line fluidly coupling a first reservoir of the reservoir system to the organ chamber, anda pumping system coupled to the reservoir system and configured to supply the first cell media from the first reservoir of the reservoir system to the organ chamber over the pulmonary arterial line to provide a fluid level in the organ chamber, the pumping system configured to drain waste media from the organ chamber to a second reservoir of the reservoir system over at least one drain line;

    a wet ventilator system configured to supply a wet ventilation to an organ airspace of the organ;

    a dry ventilator system configured to supply a dry ventilation to the organ airspace of the organ;

    a tracheal line selectively fluidly coupling the organ chamber with the reservoir system, the dry ventilator system, and the wet ventilation system; and

    a controller configured to control delivery of wet ventilation and delivery of dry ventilation, the controller configured to;

    cause seeding of the organ airspace with the second cell media via the tracheal line, cause an increase in a positive wet airway pressure provided via the tracheal line after seeding of the organ airspace, cause initiation of wet ventilation after a period of perfusion seeding of a pulmonary vasculature with the first cell media via the flow perfusion system, and switch from wet ventilation to dry ventilation after a period of wet ventilation so as to drain wet fluid from the organ airspace so as to fill the organ airspace with gas.

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