Molecular markers associated with soybean tolerance to low iron growth conditions

• US 10,059,999 B2
• Filed: 06/10/2014
• Issued: 08/28/2018
• Est. Priority Date: 06/10/2013
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Jia, Hongwu, La Vallee, Bradley, Lussenden, Roger L., Yates, Jennifer L., Ye, Xianghai
• Primary Examiner(s): Abraham, Amjad
• Assistant Examiner(s): Stankovic, Bratislav
• Application Number:
  US14/300,384
• Publication Number:
  US 20140366214A1
• Time in Patent Office: 1,540 Days
• Field of Search: None
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention provides methods and compositions for identifying soybean plants that are tolerant or have improved tolerance, or those that are susceptible to, iron deficient growth conditions. The methods use molecular markers to identify, select, and/or introgress genetic loci modulating phenotypic expression of an iron deficiency tolerance trait in soybean plant breeding. Methods are provided for screening germplasm entries for the performance and expression of this trait.

Claims

1. A method of creating a population of soybean plants with a low iron growth condition tolerant phenotype, said method comprising:

a. genotyping a first population of soybean plants or seeds;

b. detecting in said first population of soybean plants or seeds, a polymorphic nucleic acid marker linked by less than or equal to 10 cM to an allele of SEQ ID NO;

70, wherein said allele of SEQ ID NO;

70 is associated with a low iron growth condition tolerant phenotype;

c. selecting based upon said genotyping a soybean plant or seed containing said allele that was detected by genotyping;

d. crossing or selfing said selected soybean plant or a plant produced from said selected seed; and

e. producing from said crossing or selfing a population of progeny plants comprising said allele of SEQ ID NO;

70 that is associated with the low iron growth condition tolerant phenotype;

wherein said allele of SEQ ID NO;

70 comprises an Adenosine at position 201.

2. A method of claim 1, wherein said marker locus is genetically linked by less than or equal to 5 cM to SEQ ID NO:

70.

3. The method of claim 1, wherein said marker locus is in a genomic region flanked by SEQ ID NO:

70 and SEQ ID NO;

74.

4. The method of claim 1, wherein said marker locus is genetically linked by less than or equal to 1 cM to SEQ ID NO:

70.

5. The method of claim 1, wherein said marker locus is from a chromosome region flanked by any two of marker loci selected from the group consisting of SEQ ID NOs:

70 to 74.

6. The method of claim 1, wherein said marker locus is of a marker type selected from the group consisting of Restriction Fragment Length Polymorphisms (RFLP), Amplified Fragment Length Polymorphisms (AFLP), Simple Sequence Repeats (SSR), Single Nucleotide Polymorphisms (SNP), Insertion/Deletion Polymorphisms (Indels), Variable Number Tandem Repeats (VNTR), and Random Amplified Polymorphic DNA (RAPD).

7. The method of claim 1, wherein said detecting an allele comprising a method selected form the group consisting of a PCR-based detection method, a microarray method, and a nucleic acid sequencing method.

8. The method of claim 1, wherein said detecting an allele comprising a single base extension (SBE) assay.