Dual-functional nonfouling surfaces comprising target binding partner covalently coupled to polymer attached to substrate

• US 10,060,918 B2
• Filed: 12/30/2016
• Issued: 08/28/2018
• Est. Priority Date: 12/29/2006
• Status: Active Grant

• Current Assignee: University of Washington
• Original Assignee: University of Washington
• Inventors: Jiang, Shaoyi, Zhang, Zheng, Chen, Shengfu, Vaisocherova, Hana
• Primary Examiner(s): Jones, Jr., Robert
• Application Number:
  US15/396,128
• Publication Number:
  US 20170108492A1
• Time in Patent Office: 606 Days
• CPC Class Codes: C08F 122/22 Esters containing nitrogen
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/545 Synthetic resin

Abstract

Dual-functional nonfouling surfaces and materials, methods for making dual-functional nonfouling surfaces and materials, and devices that include dual-functional nonfouling surfaces and materials. The dual-functional surfaces are nonfouling surfaces that resist non-specific protein adsorption and cell adhesion. The dual-functional surfaces and materials include covalently coupled biomolecules (e.g., target binding partners) that impart specific biological activity thereto. The surfaces and materials are useful in medical diagnostics, biomaterials and bioprocessing, tissue engineering, and drug delivery.

Claims

1. A crosslinked polymer having a plurality of target binding partners covalently coupled thereto, wherein the crosslinked polymer comprises a plurality of carboxylic acid groups and a plurality of positive charged groups, wherein the crosslinked polymer is substantially electronically neutral and is a crosslinked poly(carboxybetaine) hydrogel, wherein the target binding partner is selected from the group consisting of enzymes, antibodies, antigens, receptors, and ligands, and wherein the target binding partner has affinity toward a target molecule.

2. The crosslinked polymer of claim 1, wherein the crosslinked poly(carboxbetaine) is prepared from one or more monomers selected from the group consisting of carboxybetaine acrylates, carboxybetaine acrylamides, carboxybetaine vinyl compounds, carboxybetaine epoxides, and mixtures thereof.