Longitudinal assay
First Claim
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1. A method of distinguishing specific binding of an analyte and capture agent from non-specific binding of non-analyte molecules to the capture agent, the method comprising:
- constructing a binding curve for the binding of the capture agent to an analyte, a non-analyte, or both in a sample based on a plurality of temporally-spaced detections of the capture agent within a single assay;
detecting a first detection intensity of the binding of the capture agent and a first temporal point in the binding curve at which a rise to an equilibrium has been reached or is inferred; and
if a rise to equilibrium has been reached, attributing the first detection intensity to non-specific binding;
orif a rise to equilibrium is inferred, attributing to non-specific binding a first portion of the binding curve subsequent to the first temporal point that is equal to the first detection intensity.
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Abstract
The invention relates to macro and small molecule detection and, more particularly, to methods for detecting macro and small molecules, including bio-molecules, in a liquid or gaseous sample.
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Citations
7 Claims
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1. A method of distinguishing specific binding of an analyte and capture agent from non-specific binding of non-analyte molecules to the capture agent, the method comprising:
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constructing a binding curve for the binding of the capture agent to an analyte, a non-analyte, or both in a sample based on a plurality of temporally-spaced detections of the capture agent within a single assay; detecting a first detection intensity of the binding of the capture agent and a first temporal point in the binding curve at which a rise to an equilibrium has been reached or is inferred; and if a rise to equilibrium has been reached, attributing the first detection intensity to non-specific binding;
orif a rise to equilibrium is inferred, attributing to non-specific binding a first portion of the binding curve subsequent to the first temporal point that is equal to the first detection intensity. - View Dependent Claims (2, 7)
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3. A method of distinguishing specific binding of an analyte and capture agent from non-specific binding of non-analyte molecules to the capture agent, the method comprising:
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constructing a binding curve for the binding of the capture agent to an analyte, a non-analyte, or both in a sample based on a plurality of temporally-spaced detections of the capture agent within a single assay; detecting a first detection intensity of the binding of the capture agent and a first temporal point in the binding curve at which a rise to an equilibrium has been reached; and attributing the first detection intensity to non-specific binding. - View Dependent Claims (4)
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5. A method of identifying biomarkers for therapeutic and/or diagnostic use, the method comprising:
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constructing a binding curve for the binding of a capture agent to an analyte, a non-analyte, or both in a sample based on a plurality of temporally-spaced detections of the capture agent within a single assay; detecting a first detection intensity of the capture agent and a first temporal point in the binding curve at which a rise to an equilibrium is inferred; attributing to non-specific binding a first portion of the binding curve at an additional detected intensity subsequent to the first temporal point that is equal in intensity to the first detection intensity; attributing to specific binding of the analyte a second portion of the binding curve at the additional detected intensity subsequent to the first temporal point equal to a difference between the additional detected intensity and the first detection intensity; and determining an initial concentration of the analyte based on the specific binding. - View Dependent Claims (6)
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Specification