Methods for screening patients for resistance to angioinhibition, treatment and prophylaxis thereof
First Claim
1. A method for screening a patient'"'"'s body fluid for a presence of angioinhibition resistance and treating the patient when angioinhibition resistance is identified, comprising the steps of:
- collecting a first blood or serum sample from the patient before administering an anti-antiogenic agent;
administering to the patient the anti-angiogenic agent;
collecting a second blood or serum sample from the patient after administration of the anti-angiogenic agent to the patient;
performing, on the first blood or serum sample and the second blood or serum sample, a chich chorioallantoic membrane (CAM) model estimate of angiogenesis stimulation, with the CAM configured for using the first collected blood or serum sample and the second blood or serum sample from the patient to quantify a generation of new blood vessels from existing blood vessels and new blood vessel branches;
determining from the CAM using the patient'"'"'s second blood or serum sample as a function of comparing the generation of new blood vessels and branches before and after administering the anti-angiogenic agent an estimate of the patient'"'"'s angioinhibition resistance;
and treating the patient when the estimate of the patient'"'"'s angioinhibition resistance shows that the patient has angioinhibition resistance, wherein the step of treating the patient comprises inducing a state of subclinical hypothyroidism in the patient.
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Abstract
A method for screening a patient for angioinhibition resistance and treating said patient having a disease susceptible to treatment via an anti-angiogenic agent. The screening method includes an assay for identifying the presence of angioinhibition resistance in patients by collecting patient blood or serum and subjecting it to a Chick Chorioallantoic Membrane (CAM) angiogenesis assay configured for accepting a human tumor wherein the human tumor xenograft includes a vasculature system. The screening method and assay further includes steps that include using the CAM results for identifying the endogenous pro-angiogenic non-peptide hormone concentrations of the blood sample by calculating the vascular activity of the vasculature system of the human tumor xenograft in the presence of anti-angiogenic drugs and inducing in the patient, a state of subclinical hypothyroidism prior to commencing anti-angiogenic treatment.
60 Citations
9 Claims
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1. A method for screening a patient'"'"'s body fluid for a presence of angioinhibition resistance and treating the patient when angioinhibition resistance is identified, comprising the steps of:
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collecting a first blood or serum sample from the patient before administering an anti-antiogenic agent; administering to the patient the anti-angiogenic agent; collecting a second blood or serum sample from the patient after administration of the anti-angiogenic agent to the patient; performing, on the first blood or serum sample and the second blood or serum sample, a chich chorioallantoic membrane (CAM) model estimate of angiogenesis stimulation, with the CAM configured for using the first collected blood or serum sample and the second blood or serum sample from the patient to quantify a generation of new blood vessels from existing blood vessels and new blood vessel branches; determining from the CAM using the patient'"'"'s second blood or serum sample as a function of comparing the generation of new blood vessels and branches before and after administering the anti-angiogenic agent an estimate of the patient'"'"'s angioinhibition resistance; and treating the patient when the estimate of the patient'"'"'s angioinhibition resistance shows that the patient has angioinhibition resistance, wherein the step of treating the patient comprises inducing a state of subclinical hypothyroidism in the patient. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification