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Biosensor for determining an analyte concentration

  • US 10,067,082 B2
  • Filed: 06/29/2016
  • Issued: 09/04/2018
  • Est. Priority Date: 02/06/2004
  • Status: Active Grant
First Claim
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1. A biosensor for determination of an analyte concentration in a fluid sample, the biosensor comprising:

  • a working electrode;

    a counter electrode; and

    a mixture for electrochemical reaction with an analyte, the mixture including the enzyme, a mediator, and an oxidizable species as an internal reference, the mediator being ferricyanide, the oxidizable species being ferrocyanide, the ferrocyanide being from 0.1 to 1% of the total ferricyanide, the mixture being located as a single layer directly on a surface of at least a portion of the working electrode prior to the introduction of the fluid sample, the mixture including only one enzyme.

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