Biosensor for determining an analyte concentration
First Claim
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1. A biosensor for determination of an analyte concentration in a fluid sample, the biosensor comprising:
- a working electrode;
a counter electrode; and
a mixture for electrochemical reaction with an analyte, the mixture including the enzyme, a mediator, and an oxidizable species as an internal reference, the mediator being ferricyanide, the oxidizable species being ferrocyanide, the ferrocyanide being from 0.1 to 1% of the total ferricyanide, the mixture being located as a single layer directly on a surface of at least a portion of the working electrode prior to the introduction of the fluid sample, the mixture including only one enzyme.
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Abstract
A biosensor (102) for determining the presence or amount of a substance in a sample and methods of use of the biosensor (102) are provided. The biosensor (102) for receiving a user sample to be analyzed includes a mixture for electrochemical reaction with an analyte. The mixture includes an enzyme, a mediator and an oxidizable species as an internal reference.
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Citations
9 Claims
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1. A biosensor for determination of an analyte concentration in a fluid sample, the biosensor comprising:
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a working electrode; a counter electrode; and a mixture for electrochemical reaction with an analyte, the mixture including the enzyme, a mediator, and an oxidizable species as an internal reference, the mediator being ferricyanide, the oxidizable species being ferrocyanide, the ferrocyanide being from 0.1 to 1% of the total ferricyanide, the mixture being located as a single layer directly on a surface of at least a portion of the working electrode prior to the introduction of the fluid sample, the mixture including only one enzyme. - View Dependent Claims (2, 3)
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4. A method of forming and applying a reagent mixture for an electrochemical reaction with an analyte of a fluid sample in a biosensor, the biosensor having a working electrode and a counter electrode, the method the acts of comprising:
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forming a batch of the reagent mixture by adding an enzyme, adding a mediator and adding a known amount of an oxidizable species, the added oxidizable species being added separately from the mediator, the mediator being ferricyanide, the oxidizable species being ferrocyanide; and after forming the reagent mixture, placing the reagent mixture directly on a surface of at least a portion of the working electrode of the biosensor prior to the introduction of the fluid sample, the mixture including only one enzyme. - View Dependent Claims (5, 6)
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7. A method of using a biosensor to assist in determining glucose concentration of a fluid sample, the method comprising:
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providing a biosensor including a working electrode, a counter electrode and a reagent, the reagent located directly on a surface of at least a portion of the working electrode prior to introduction of the test sample, the reagent including an enzyme, a mediator and an oxidizable species as an internal reference, the mediator being ferricyanide, the oxidizable species being ferrocyanide, the ferrocyanide being from 0.1 to 1% of the total ferricyanide; applying a first voltage potential in a first voltage period; providing a set delay period; and applying a second voltage potential in a second voltage period following the delay period. - View Dependent Claims (8, 9)
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Specification