Electrochemical sensor array and apparatus

• US 10,067,083 B2
• Filed: 04/06/2015
• Issued: 09/04/2018
• Est. Priority Date: 04/07/2014
• Status: Active Grant

• Current Assignee: Tubitak
• Original Assignee: Tubitak
• Inventors: Uludag, Yildiz, Sagiroglu, Mahmut Samil, Ersoy, Aylin, Ardinc, Edis, Budak, Sinan, Demiralp, Atike, Efe, Halit Hakan
• Primary Examiner(s): Kaur, Gurpreet
• Assistant Examiner(s): Rosenwald, Steven Eric
• Application Number:
  US15/302,557
• Publication Number:
  US 20170030855A1
• Time in Patent Office: 1,247 Days
• Field of Search: None
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 27/27 Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68

Abstract

This invention relates to an apparatus suitable to be used for analyzing at least one sample with an electrochemical sensor array, comprising at least one upper layer; at least one inlet and at least one outlet opening provided on the upper layer; at least one lower layer having at least one recess thereon; at least one two-sided adhesive membrane matching to the recess; at least one sensor array secured to the lower layer, at least one reference electrode, at least one counter electrode, at least one communication channel, at least one conductive line providing a connection between the working electrode and the communication channel on at least one plate; at least one further two-sided adhesive membrane spaced from the adhesive membrane positioned on the lower layer and enabling the sensor array to be secured to the upper layer.

Claims

1. An apparatus having an electrochemical sensor array for analyzing a sample comprising:

at least one upper layer;

at least one inlet opening and at least one outlet opening, wherein the inlet opening and the outlet opening are arranged on the upper layer;

at least one lower layer having a recess thereon;

at least one first two-sided adhesive membrane matching to the recess on the lower layer;

at least one sensor array secured to the lower layer and embedded within the recess through the first two-sided adhesive membrane, wherein the sensor array includes at least one working electrode, at least one reference electrode, at least one counter electrode, at least one communication channel, and at least one conductive line, wherein the conductive line provides a connection between the working electrode and the communication channel;

wherein the working electrode, the reference electrode, and the counter electrode are integrally formed on an upper surface of a plate;

at least one measuring device associated with the communication channel;

at least one second two-sided adhesive membrane spaced from the first two-sided adhesive membrane, wherein the second two-sided adhesive membrane is provided between the sensor array and the lower layer, such that the sensor array is secured to the upper layer, the second two-sided adhesive membrane is placed adjacent to the upper layer and is provided with an opening;

at least one flow channel formed between the upper layer and the sensor array as a result of the opening wherein the flow channel is associated with the inlet opening and the outlet opening located on the upper layer for carrying the sample.

2. The apparatus according to claim 1, wherein the plate is made of glass, silicon dioxide or ceramic.

3. The apparatus according to claim 1, wherein each of the working electrode, the reference electrode, and the counter electrode is made of gold, titanium, platinum, chromium or aluminum.

4. The apparatus according to claim 1, wherein each of the upper layer and the lower layer is made of plastic.

5. The apparatus according to claim 1, wherein three working electrodes are arranged on the plate.

6. The apparatus according to claim 1, wherein a plurality of working electrodes are arranged on the plate.

7. The apparatus according to claim 1, wherein a plurality of working electrodes are arranged on the plate, and the plurality of working electrodes are spaced from each other.

8. The apparatus according to claim 7, wherein the reference electrode, the counter electrode and the working electrode are aligned in order to receive a same electrochemical reaction from each working electrode throughout an analysis.

9. The apparatus according to claim 1, wherein the upper layer has a size smaller than that of the lower layer to allow the conductive line not to be covered and to connect the conductive line to the measuring device.

10. The apparatus according to claim 1, wherein the reference electrode and the counter electrode form a rectangular enclosure around the working electrodes.

11. The apparatus according to claim 10, wherein the reference electrode and the counter electrode are L-shaped and positioned in two opposite sides of the working electrodes, wherein a lateral line of L-shaped reference electrode extends toward the counter electrode, a lateral line of the L-shaped counter electrode extends toward the reference electrode, and the lateral line of the L-shaped counter electrode is located above and covers a part of the lateral line of L-shaped reference electrode.

12. The apparatus according to claim 1, wherein each of the upper layer and the lower layer comprises at least one hole matching to each other so that the upper layer is reliably secured to the lower layer through at least one connecting member.

13. The apparatus according to claim 1, wherein the working electrode has a diameter larger than the width of the conductive line.

14. The apparatus according to claim 2, wherein the working electrode, the reference electrode, and the counter electrode are made of gold, titanium, platinum, chromium or aluminum.

15. The apparatus according to claim 2, wherein at least one of the upper layer and the lower layer is made of plastic.

16. The apparatus according to claim 3, wherein at least one of the upper layer and the lower layer is made of plastic.

17. The apparatus according to claim 14, wherein the upper layer and the lower layer are made of plastic.

18. The apparatus according to claim 1, wherein the upper layer or the lower layer is made of plastic.