Compartmental extract compositions for tissue engineering

• US 10,071,185 B2
• Filed: 02/08/2009
• Issued: 09/11/2018
• Est. Priority Date: 02/07/2008
• Status: Active Grant

• Current Assignee: Nayacure Therapeutics Ltd.
• Original Assignee: Nayacure Therapeutics Ltd.
• Inventors: Cohen, Shahar
• Primary Examiner(s): EPPS -SMITH, JANET L
• Application Number:
  US12/866,627
• Publication Number:
  US 20110008397A1
• Time in Patent Office: 3,502 Days
• Field of Search: 424400, 424520, 424725, 424 937, 435536
• CPC Class Codes: A61K 35/00 Medicinal preparations containing materials or reaction products thereof with undetermined constitution
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 27/3604 characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12M 23/26 flexible flexible containers for laboratory use B01L3/505
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Abstract

The present invention concerns biologically-active cell-free scaffolds composed of extracts of cellular and/or extracellular compartments for use in tissue regeneration. The present invention also contemplates the novel concept of redesigning the biological scaffold by seeding cells thereon followed by cell elimination. Cells are seeded on the scaffold for a period of time during which a dynamic interaction occurs between the scaffold and the seeded cells, resulting in reshaping of the scaffold architecture and integration of newly synthesized matrix elements. Redesigning may improve the physical and biological characteristics of the scaffold, and also improve the matching of the scaffold to treat a specific target tissue or a specific patient, by seeding tissue-specific cells or by seeding cells which are autologous to a patient, respectively.

Claims

1. A cell free scaffold having a three dimensional structure and a surface suitable for adherence and proliferation of cells and composed of an extract of cells, wherein the extract consists of extracellular components secreted by, and/or intracellular components extracted from, cells isolated and cultured in vitro, and wherein the scaffold is formulated in injectable form or fabricated in transplantable form, and wherein said scaffold is not fabricated by electroprocessing or electrospinning.

2. The scaffold of claim 1, wherein said extract of cells is prepared from a cell selected from the group consisting of a primary cell and a cell line, and wherein the cell is selected from the group consisting of an animal cell, a mammalian cell, a human cell and a plant cell.

3. The scaffold of claim 1, wherein said scaffold is suitable for administration into a mammal for use in conditions necessitating tissue or organ regeneration, repair or replacement.

4. The scaffold of claim 1, further comprising a scaffold-enhancing agent or an additive.

5. The scaffold of claim 3, wherein said scaffold is seeded with cells that adhere to the scaffold.

6. The scaffold of claim 1, wherein the extract is from cells selected from the group consisting of fibroblasts, keratinocytes, epithelial cells, endothelial cells, embryonic stem cells, somatic cells, pluripotent cells, neural cells, epidermal cells, hematopoietic cells, melanocytes, chondrocytes, hepatocytes, B-cells, T-cells, erythrocytes, macrophages, monocytes, muscle cells, vascular smooth muscle cells, stem cells, differentiated cells, plant cells, mammalian cells, mesenchymal cells, oral and gastrointestinal mucosal epithelia cells, urinary tract epithelia cells, vascular endothelial cells, neural cells, epidermal cells, osteoblasts, intervertebral disc cells, pancreatic cells, angioblasts, bone marrow, mesenchymal cells, myoblasts, cardiomyocytes, amniotic cells, placental cells, genetically engineered cells and combinations thereof.

7. The scaffold of claim 5, wherein the scaffold is redesigned by the adherent cells, and wherein redesigning refers to structural and optionally functional modification of the scaffold by the adherent cells.

8. The scaffold of claim 7, wherein the adherent cells are eliminated from the scaffold.

9. The scaffold of claim 7, wherein the scaffold is structurally modified by at least one cycle of cell seeding and elimination.

10. The scaffold of claim 1, wherein the extract of cells is compartmentalized or fractionated.

11. The scaffold of claim 1, wherein the scaffold is fabricated by a fabrication process selected from the group consisting of solvent-casting and particulate-leaching, gas foaming, fiber meshes/fiber bonding, filament drawing, phase separation, melt molding, compression molding, emulsion freeze drying, solution casting, coating, weaving, and freeze drying.

22. A method of providing support to a tissue, or treating conditions necessitating tissue or organ regeneration, repair or replacement in a patient, comprising administering the scaffold of claim 1 in proximity to said tissue.

12. A method of preparing a cell free scaffold having three dimensional structure and a surface suitable for adherence and proliferation of cells for use in conditions necessitating tissue or organ regeneration, repair or replacement, comprising:

(a) isolating cells and culturing said cells in vitro;

(b) preparing a cell extract, wherein the extract consists of extracellular components secreted by, and/or intracellular components extracted from said cultured cells; and

(c) preparing a scaffold from said cell extract, wherein the scaffold is formulated in injectable form or fabricated in transplantable form, and wherein said scaffold is not fabricated by electroprocessing or electro spinning.

13. The method according to claim 12, wherein said cell extract is prepared from a cell selected from the group consisting of:

a primary cell and a cell line, and wherein the cell is selected from the group consisting of;

an animal cell, a mammalian cell, a human cell and a plant cell.

14. The method according to claim 12, wherein the extracellular components secreted by, and/or intracellular components extracted from said cultured cells are selected from the group consisting of a cytosolic extract, a cytoplasmic extract, a nuclear extract, a whole cell lysate, extracellular extract, and mixtures thereof.

15. The method according to claim 12, wherein said isolated cells are cultured in a cell culture device capable of exerting mechanical forces onto the cultured cells and having a patterned surface.

16. The method according to claim 12, wherein said scaffold is suitable for administration to a mammal for use in conditions necessitating tissue or organ regeneration, repair or replacement.

17. The method according to claim 12, further comprising a scaffold-enhancing agent or an additive.

18. The method according to claim 16, wherein said method further comprises seeding the scaffold with cells that adhere to the scaffold prior to administration.

19. The method according to claim 18, wherein said method further comprises eliminating said seeded cells from the scaffold prior to administration.

20. The method according to claim 19, wherein said scaffold is redesigned by at least one cycle of cell seeding and elimination.

21. The method according to claim 20, wherein said scaffold is seeded with more than one type of cells.