Spectrometric systems and mechanical methods for improved focus localization of time and space-varying measurements

• US 10,072,983 B2
• Filed: 07/06/2017
• Issued: 09/11/2018
• Est. Priority Date: 04/30/2012
• Status: Active Grant

• Current Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Original Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Inventors: Haider, Clifton R., Rose, James A., Delp, Gary S., Gilbert, Barry K.
• Primary Examiner(s): Ahmed, Jamil
• Application Number:
  US15/643,193
• Publication Number:
  US 20170322084A1
• Time in Patent Office: 432 Days
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14552 Details of sensors specially adapted therefor
• CPC Class Codes: G01J 3/0237 Adjustable, e.g. focussing
• CPC Class Codes: G01J 3/42 Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry beam switching arrangements G01J3/08
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: G01N 33/4925 measuring blood gas content, e.g. O2, CO2 , HCO3

Abstract

A system and method of dynamically localizing a measurement of parameter characterizing tissue sample with waves produced by spectrometric system at multiple wavelengths and detected at a fixed location of the detector of the system. The parameter is calculated based on impulse response of the sample, reference data representing characteristics of material components of the sample, and path lengths through the sample corresponding to different wavelengths. Dynamic localization is effectuated by considering different portions of a curve representing the determined parameter, and provides for the formation of a spatial map of distribution of the parameter across the sample. Additional measurement of impulse response at multiple detectors facilitates determination of change of the measured parameter across the sample as a function of time.

Claims

1. An apparatus for collection of optical data, the apparatus comprising:

a source of light including at least one transmitter configured to emit a beam of first light at at least one corresponding wavelength along a first axis;

a device including

a first set of selectively and individually re-orientable reflecting elements disposed parallel to a first plane when each of said reflecting elements from the first set is in a first reference orientation;

a reflecting element from the first set configured to redirect the first light, received from said beam, towards a predetermined point,

wherein the predetermined point is separate from the first set;

an optical receiver disposed in optical communication with said device to receive second light, the second light being the first light that has been scattered at the predetermined point;

and

a computer-readable processor and tangible, non-transitory storage medium containing program code thereon which, when downloaded onto the processor, causes the processor

to receive reference data representing an empirically-defined spectrally-dependent characteristic of an object present at said predetermined point;

to determine an impulse response of the object, the impulse response being associated with emission of the first light by the source; and

to determine, as a function of time, at least one of (i) a wavelength-dependent material parameter characterizing the object and (ii) a position-dependent parameter characterizing the object, based on the determined impulse response and the reference data.

2. An apparatus according to claim 1, further comprising a second set of selectively and individually re-orientable reflecting elements disposed parallel to a second plane when each of said reflecting elements from the second set is in a second reference orientation, the second plane being transverse to the first plane and substantially parallel to the first axis.

3. An apparatus according to claim 2, wherein a reflecting element from the second set is configured to receive said second light and to redirect the second light towards the optical receiver.

4. An apparatus according to claim 2, configured to vary an optical path associated with propagation of the first light from the reflecting element from the first set to the predetermined point and then to the reflecting element from the second set as a result of spatially reorienting at least one of (i) the reflecting element from the first set and (ii) the reflecting element from the second set.

5. An apparatus according to claim 1, wherein the device comprises a baffle disposed to prevent said optical receiver from i) receiving said first light that has not been scattered at the predetermined point and from ii) receiving said second light that has interacted with the reflecting element from the first set.

6. An apparatus according to claim 1, configured to satisfy at least one of the following conditions:

(i) different reflecting elements of the first set are configured to be reorientable synchronously with one another; and

(ii) different reflecting elements of the second set are configured to be reorientable synchronously with one another.

7. An apparatus according to claim 1, wherein said predetermined point is a point at a surface of an optical disk.

8. An apparatus according to claim 1, configured to vary an optical path associated with propagation of the first light from the reflecting element from the first set through the predetermined point to the optical detector as a result of spatially reorienting the reflecting element from the first set.

9. An apparatus according to claim 2, wherein at least one of the first and second sets includes only one, single reflecting element.

10. An apparatus according to claim 2, wherein the first and second sets includes reflecting elements disposed in respectively-corresponding first and second rows.

11. An apparatus according to claim 2, wherein first and second sets of reflecting elements are portions of a two-dimensional array of reflecting elements.

12. An apparatus according to claim 2, wherein the device is configured to reposition all reflecting elements from both the first and second sets to assume the second reference orientation.

13. An apparatus according to claim 2, wherein the device is configured to spatially-continuously vary an angle formed by the first and second planes in response to changing at least one of the first and second orientations.

14. An apparatus according to claim 2, wherein the device is configured to change orientation, of at least one of the reflecting elements of the first set and the reflecting elements of the second set, in an angularly-discrete fashion.

15. An apparatus for collection of optical data, the apparatus comprising:

an optical detector;

a source of light including at least one transmitter configured to emit a beam of first light at at least one corresponding wavelength along a first axis;

an optical system containing an array of spatially-reorientable reflecting elements and configured to deliver the first light, emanating from the source of light, to the optical detector while scanning an object with the beam by reorienting first and second groups of said reflecting elements between first and second reference orientations, respectively;

and

a data-processing system in operable communication with the optical detector and configured to acquire an output produced by the optical detector in response to being irradiated with said first light that has been scattered by the object, the data-processing system including a computer-readable processor and tangible, non-transitory storage medium containing program code thereon,

wherein said program code, when loaded onto the processor, causes the processor

to receive reference data representing an empirically-defined spectrally-dependent characteristic of the object;

to determine an impulse response of the object, the impulse response being associated with emission of the beam of light by the source; and

to determine, as a function of time, at least one of (i) a wavelength-dependent material parameter characterizing the object and (ii) a position-dependent parameter characterizing the object, based on a determined impulse response and the reference data

when the object is positioned at an intermediate point along a direction of propagation of the beam between the first and second groups of the reflecting elements,

wherein the first group of reflecting elements is positioned to receive the beam from the source of light,

wherein the second group of reflecting elements is configured to redirect the first light, that has been scattered by the object, towards the optical detector.

16. An apparatus according to claim 15, wherein the device is configured to spatially-continuously reorient reflecting elements from at least one of the first and second groups between the first and second reference orientations, a surface of the reflecting element in the first orientation being transverse to the first axis, a surface of the reflecting element in the second orientation being parallel to the first axis.

17. An apparatus according to claim 15, further comprising a baffle disposed in a first plane to block the first light from propagating directly between the source and the optical detector, wherein the first plane is perpendicular to a plane defined when all reflecting elements of the optical system are in the second reference orientation.

18. An apparatus according to claim 16,

configured for use with the object that includes a biological tissue, and

wherein data-processing system is configured to determine an optical property of the object without the use of an arterial pulse in the object,

the optical property including at least one of a) wavelength-dependent scattering, b) absorption, and c) anisotropy of the object, said optical property having been determined without the use of an arterial pulse in the sample.

19. An apparatus according to claim 17, wherein the data-processing system is configured to determine the at least one wavelength-dependent material parameter characterizing the object based on an averaged wavelength-dependent path length that has been estimated with the use of the determined impulse response and scattering of light from said beam in the object, said averaged wavelength dependent path being different from a straight path between a reflecting element from the first group and a reflecting element of the second group.

20. An apparatus according to claim 19, wherein the data-processing system is further configured

to sample a curve representing the determined wavelength-dependent material parameter as a function of time to obtain values of said material parameter at sampling points in time, and

to form a weighting function by calculating variance values among the values of said material parameter at the sampling points in time, the weighting function being defined by said variance values as a function of time.

21. An apparatus according to claim 20, wherein the data-processing system is further configured to modify the determined wavelength-dependent material parameter characterizing the object based on (i) the computed impulse response that has been weighted by the weighting function and (ii) the reference data.

22. A method for collecting optical data, the method comprising:

redirecting a beam of light, that has been received from an optical source, with a first spatially-reorientable reflecting element from an array of reflecting elements of an optical device, towards a first point of an object,

wherein said beam of light is characterized by at least one wavelength;

receiving first light from said beam, that has been scattered by the object, from a second point of the object with a second spatially-reorientable reflecting element from said array to redirect said first light towards an optical detector; and

with a data-processing system including a computer-readable processor;

acquiring an output generated by the optical detector in response to being irradiated with said first light, the output representing an optical property of the object determined along a path from said first reflecting element to said second reflecting element through the object, said path defined by a gradient of concentration of a material component in the object;

receiving, with the data-processing unit of the system, reference data representing an empirically-defined spectrally-dependent characteristic of the object;

determining an impulse response of the object, the impulse response being associated with emission of light from the optical source; and

determining, as a function of time, at least one of (i) a wavelength-dependent material parameter characterizing the object and (ii) a position-dependent material parameter characterizing the object, based on a determined impulse response and the reference data.

23. A method according to claim 22,

wherein the object includes biological tissue, and

wherein the acquiring the output includes acquiring the output representing the optical property including at least one of a) wavelength-dependent scattering, b) absorption, and c) anisotropy of the object, said optical property having been determined without the use of an arterial pulse in the object.

24. A method according to claim 22,

wherein the determining, as a function of time, the wavelength-dependent material parameter characterizing the object includes determining said wavelength-dependent material parameter based on an averaged wavelength-dependent path length that has been estimated with the use of the determined impulse response and scattering in the object,

wherein said averaged wavelength dependent path is different from a straight path between the first and second points of the object.

25. A method according to claim 24, further comprising:

with the data-processing system, sampling a curve representing the determined wavelength-dependent material parameter as a function of time to obtain values of said parameter at sampling points in time, and

forming a weighting function by calculating variance values among the values of said determined wavelength-dependent material parameter at sampling points in time, the weighting function being defined by said variance values as a function of time.

26. A method according to claim 25, further comprising:

modifying the determined wavelength-dependent material parameter characterizing the object based on (i) a computed impulse response that has been weighted by the weighting function and (ii) the reference data.

27. A method according to claim 22,

wherein the object includes biological tissue;

and

wherein said determining the wavelength-dependent material parameter includes determining one or more of a level of oxygenation of the object and a concentration of a chosen species in the object,

and further comprising;

at different wavelengths, modulating said beam of light with first and second excitation sequences applied to the source of light, the first and second excitation sequences being orthogonal to one another.

28. A method according to claim 22,

wherein the determining the impulse response includes determining the impulse response of the object based on the output, the output being a time-dependent characteristic of a pair formed by the source of light and the optical receiver, the pair identified at the data-processing unit of the system by a respectively corresponding excitation sequence series of pulses produced by the source of light, a first excitation sequence series of pulses at a first wavelength being orthogonal to a second excitation sequence series of pulses at a second wavelength.

29. A method according to claim 22,

wherein the object includes a biological tissue;

and

further comprising adding a chemical substance to the object; and

wherein said determining the wavelength-dependent material parameter includes determining a change in the wavelength-dependent material parameter caused by said adding.

30. A method according to claim 29, further comprising generating said beam of light as electromagnetic pulses in a defined excitation sequence and transmitting said pulses through a delay line with multiple delay taps

to enhance said determining the change in the at least one wavelength-dependent material parameter and to reduce a cost of the determining of said impulse response.

31. A method according to claim 22, wherein the object includes a biological sample, and further comprising

repositioning at least one of the source of light and the optical detector along a surface of the biological sample to enhance localization of said determining the wavelength-dependent material parameter based on an image of the biological sample created as a result of said repositioning, the image representing a structure of the biological sample.

32. A method according to claim 22, wherein the object is homogeneous.

33. A method according to claim 22, further comprising blocking light from said beam of light from propagating directly from the source of light to the optical detector along a surface of said array.