Microfluidic devices, and methods of making and using the same
First Claim
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1. An assay method using a microfluidic device;
- the method comprising;
(a) providing a microfluidic device comprising;
a sample application site;
a flow channel in fluid communication with the sample application site; and
a porous component positioned between the sample application site and flow channel, wherein the porous component comprises a porous matrix comprising pores and an assay reagent comprising an analyte-specific optically detectable label positioned within the pores of the porous matrix;
(b) contacting a sample to the sample application site so that the sample flows through the porous matrix and into the flow channel, wherein the porous matrix is configured to mix the sample with the assay reagent to produce a labelled sample that then flows into the flow channel;
(c) illuminating the labelled sample in the flow channel with a light source; and
(d) detecting light from the labelled sample.
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Abstract
The present disclosure provides methods and systems for assaying a sample. A microfluidic device to perform an assay of a sample (e.g., biological sample) is described having a sample application site, a porous component and a flow channel. The porous component provides for uniform dissolution of a reagent and mixing of the sample and reagent without filtering the sample.
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20 Claims
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1. An assay method using a microfluidic device;
- the method comprising;
(a) providing a microfluidic device comprising; a sample application site; a flow channel in fluid communication with the sample application site; and a porous component positioned between the sample application site and flow channel, wherein the porous component comprises a porous matrix comprising pores and an assay reagent comprising an analyte-specific optically detectable label positioned within the pores of the porous matrix; (b) contacting a sample to the sample application site so that the sample flows through the porous matrix and into the flow channel, wherein the porous matrix is configured to mix the sample with the assay reagent to produce a labelled sample that then flows into the flow channel; (c) illuminating the labelled sample in the flow channel with a light source; and (d) detecting light from the labelled sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
- the method comprising;
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18. An assay method using a microfluidic device;
- the method comprising;
contacting a sample to a sample application site of a microfluidic device, the microfluidic device comprising; a flow channel in fluid communication with the sample application site; and a porous component positioned between the sample application site and flow channel, wherein the porous component comprises a porous matrix and an assay reagent; wherein the porous matrix comprises pores;
wherein the assay reagent is positioned within the pores of the porous reagent; andwherein the porous matrix is configured to mix the sample with the assay reagent to produce a labeled sample that flows into the flow channel. - View Dependent Claims (19, 20)
- the method comprising;
Specification