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Systems and methods for reagent storage

  • US 10,076,751 B2
  • Filed: 12/30/2013
  • Issued: 09/18/2018
  • Est. Priority Date: 12/30/2013
  • Status: Active Grant
First Claim
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1. A microfluidic device for storing a reagent, comprising:

  • a single unit, comprising;

    a first portion of the microfluidic device comprising a reagent storage compartment configured to hold a reagent;

    a second portion of the microfludic device comprising a reaction chamber configured to receive the reagent and to support the reagent during a reaction process to form a product; and

    a valve comprising a membrane, wherein the valve is located downstream of the reagent storage compartment and upstream of the reaction chamber relative to a flow path extending between the reagent storage and the reaction chamber, and the valve is configured to isolate the reagent storage compartment from the reaction chamber when the valve is in a closed state; and

    a sealing mechanism positioned over the valve, wherein the sealing mechanism comprises a support structure and a clamp extending from the support structure that is configured to contact the membrane and to depress the membrane to cause the valve to reach the closed state, wherein the sealing mechanism comprises arms extending from the support structure that flank the clamp, and the sealing mechanism is positioned over the valve such that the support structure is proximate to a first surface of the device, and the arms extending from the support structure are configured to extend into the device and via protrusions on ends of the arms to engage a second surface of the device at a location underneath the valve to hold the valve in the closed state.

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