Systems and methods for reagent storage

• US 10,076,751 B2
• Filed: 12/30/2013
• Issued: 09/18/2018
• Est. Priority Date: 12/30/2013
• Status: Active Grant

• Current Assignee: General Electric Company
• Original Assignee: General Electric Company
• Inventors: Samper, Victor Donald, Rensch, Christian Friedrich Peter, Boeld, Christoph, Salvamoser, Ruben Julian
• Primary Examiner(s): White, Dennis
• Assistant Examiner(s): Kilpatrick, Bryan
• Application Number:
  US14/144,047
• Publication Number:
  US 20150182963A1
• Time in Patent Office: 1,723 Days
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00783 Laminate assemblies, i.e. the reactor comprising a stack of plates
• CPC Class Codes: B01J 2219/00873 Heat exchange
• CPC Class Codes: B01J 2219/00889 Mixing
• CPC Class Codes: B01J 2219/00894 More than two inlets
• CPC Class Codes: B01J 2219/00961 Temperature
• CPC Class Codes: B01J 2219/00963 Pressure
• CPC Class Codes: B01J 2219/00966 pH
• CPC Class Codes: B01J 2219/00986 Microprocessor
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/523 with means for closing or opening
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 99/0015 : Diaphragm or membrane valves
• CPC Class Codes: F16K 99/003 : Valves for single use only
• CPC Class Codes: Y10T 156/10 : Methods of surface bonding and/or assembly therefor

Abstract

A microfluidic device for storing a reagent includes a single unit includes a first portion having a reagent storage chamber configured to hold a reagent. The device also includes a second portion having a reaction chamber configured to support the reagent during a reaction process to form a product. The device also includes a valve configured to isolate the reagent storage compartment from the reaction chamber when the valve is in a closed state.

Claims

1. A microfluidic device for storing a reagent, comprising:

a single unit, comprising;

a first portion of the microfluidic device comprising a reagent storage compartment configured to hold a reagent;

a second portion of the microfludic device comprising a reaction chamber configured to receive the reagent and to support the reagent during a reaction process to form a product; and

a valve comprising a membrane, wherein the valve is located downstream of the reagent storage compartment and upstream of the reaction chamber relative to a flow path extending between the reagent storage and the reaction chamber, and the valve is configured to isolate the reagent storage compartment from the reaction chamber when the valve is in a closed state; and

a sealing mechanism positioned over the valve, wherein the sealing mechanism comprises a support structure and a clamp extending from the support structure that is configured to contact the membrane and to depress the membrane to cause the valve to reach the closed state, wherein the sealing mechanism comprises arms extending from the support structure that flank the clamp, and the sealing mechanism is positioned over the valve such that the support structure is proximate to a first surface of the device, and the arms extending from the support structure are configured to extend into the device and via protrusions on ends of the arms to engage a second surface of the device at a location underneath the valve to hold the valve in the closed state.

2. The microfluidic device of claim 1, wherein the microfluidic device is configured to store and to isolate all reagents utilized in the reaction process to form the product from a surrounding environment for a period of time prior to initiation of the reaction process.

3. The microfluidic device of claim 1, wherein the sealing mechanism is configured to enable the valve to be in an open state only when at least a portion of the sealing mechanism is broken.

4. The microfluidic device of claim 1, wherein the first portion and the second portion are coupled together to form the single unit.

5. The microfluidic device of claim 1, wherein the first portion includes a cavity configured to removably receive the second portion.

6. The microfluidic device of claim 1, comprising a coating disposed within the reagent storage compartment configured to reduce diffusion of the reagent from the reagent storage compartment.

7. The microfluidic device of claim 1, wherein the first portion comprises at least two reagent storage compartments each storing different reagents.

8. The microfluidic device of claim 1, comprising the reagent within the reagent storage compartment, wherein the reagent is configured to generate a PET or a SPECT tracer.

9. A system for storing a reagent, comprising:

a microfluidic device comprising;

one or more layers forming a substrate;

a reagent storage compartment configured to store a reagent and disposed within the substrate;

a reaction chamber fluidly coupled to the reagent storage compartment via a flow path extending between the reagent storage compartment and the reaction chamber, wherein the reaction chamber is configured to hold the reagent during a reaction process;

a valve disposed across the flow path downstream of the reagent storage compartment and upstream of the reaction chamber, wherein the valve comprises a membrane and is configured to isolate the reagent storage compartment from the reaction chamber when the valve is in a closed state;

a sealing mechanism configured to be positioned over the valve, wherein the sealing mechanism comprises a support structure and a clamp extending from the support structure that is configured to contact the membrane and to depress the membrane to cause the valve to reach the closed state, wherein the sealing mechanism comprises arms extending from the support structure that flank the clamp, and the sealing mechanism is positioned over the valve such that the support structure is proximate to a first surface of the device, and the arms extending from the support structure are configured to extend into the device and via protrusions on ends of the arms to engage a second surface of the device at a location underneath the valve to hold the valve in the closed state; and

an interface system configured to manipulate the sealing mechanism to initiate the movement of the reagent from the reagent storage compartment toward the reaction chamber.

10. The system of claim 9, wherein the interface system is configured to break at least a portion of the sealing mechanism to adjust the valve to an open state to initiate the movement of the reagent from the reagent storage compartment toward the reaction chamber.

11. The system of claim 9, wherein the device comprises the reagent, wherein the system is configured to synthesize a PET tracer or a SPECT tracer using the reagent.

12. The system of claim 9, wherein the device comprises a first portion having the reaction storage compartment and a second portion having the reaction chamber, wherein the first portion is removably coupled to the second portion.

13. A method of manufacturing a microfluidic device for storing a reagent, comprising:

generating a substrate having one or more layers;

creating a reagent storage compartment within the substrate, the reagent storage compartment having an inlet and a flow path configured to transport a reagent from the reagent storage compartment;

coupling one or more additional layers having a reaction chamber formed therein to the flow path;

inserting a reagent into the reagent storage compartment via the inlet;

applying a valve having a valve body and a membrane along the flow path downstream of the reagent storage compartment and upstream of the reaction chamber; and

positioning a sealing mechanism comprising a clamp over the membrane;

moving the sealing mechanism relative to the valve to cause the clamp to contact and to depress the membrane to seal the flow path and to contain the reagent within the reagent storage compartment, and

mechanically locking the sealing mechanism about the valve by driving arms of the sealing mechanism that flank the clamp through the valve body from a first side of the valve body to a second side of the valve body to enable protrusions on ends of the arms to engage a surface on the second side of the valve body located underneath the valve body.

14. The method of claim 13, comprising bonding the one or more additional layers to the one or more layers.

15. The method of claim 13, comprising applying a coating to at least a portion of an interior of the reagent storage compartment, the coating being configured to reduce diffusion of the reagent out of the reagent storage component.

16. The method of claim 13, wherein the stored reagent is configured to be used to synthesize a PET tracer.