Methods of producing RPE cells and compositions of RPE cells
First Claim
1. A method of producing retinal pigment epithelial (RPE) cells, comprising(a) culturing human pluripotent stem cells for 7-14 days to form embryoid bodies or aggregates in suspension culture in a medium that comprises less than 5% animal-derived protein;
- (b) culturing the embryoid bodies or aggregates as an adherent culture in a medium that comprises less than 5% animal-derived protein, wherein RPE cells appear within 14-28 days of adherent culture;
(c) selecting RPE cells from the culture of step (b);
(d) culturing the RPE cells selected in step (c) thereby producing a culture comprising RPE cells.
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Abstract
The present invention provides improved methods for producing RPE cells from human embryonic stem cells or from other human pluripotent stem cells. The invention also relates to human retinal pigmented epithelial cells derived from human embryonic stem cells or other human multipotent or pluripotent stem cells. hRPE cells derived from embryonic stem cells are molecularly distinct from adult and fetal-derived RPE cells, and are also distinct from embryonic stem cells. The hRPE cells described herein are useful for treating retinal degenerative diseases.
70 Citations
13 Claims
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1. A method of producing retinal pigment epithelial (RPE) cells, comprising
(a) culturing human pluripotent stem cells for 7-14 days to form embryoid bodies or aggregates in suspension culture in a medium that comprises less than 5% animal-derived protein; -
(b) culturing the embryoid bodies or aggregates as an adherent culture in a medium that comprises less than 5% animal-derived protein, wherein RPE cells appear within 14-28 days of adherent culture; (c) selecting RPE cells from the culture of step (b); (d) culturing the RPE cells selected in step (c) thereby producing a culture comprising RPE cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification