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Method for the synthesis of a bifunctional complex

  • US 10,077,440 B2
  • Filed: 07/08/2015
  • Issued: 09/18/2018
  • Est. Priority Date: 10/30/2002
  • Status: Expired due to Term
First Claim
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1. A method for the synthesis of a library of different bifunctional complexes, each comprising a molecule part and a double stranded oligonucleotide coding part identifying the molecule part, said method comprising the steps ofi) providing a scaffold “

  • X”

    linked to an oligonucleotide “

    x”

    , wherein the oligonucleotide “

    x”

    is capable of functioning as an identifier for scaffold “

    X”

    in a plurality of different reaction compartments;

    ii) providing a plurality of different reactants “

    A”

    ;

    iii) providing a plurality of different oligonucleotides “

    a”

    , wherein the different oligonucleotides “

    a”

    are capable of functioning as identifiers for the different reactants “

    A”

    ;

    iv) reacting a different reactant “

    A”

    from step (ii) with scaffold “

    X”

    in each of the different reaction compartments, thereby generating a plurality of different molecule parts “

    XA”

    ;

    v) connecting a different oligonucleotide “

    a”

    from step (iii) with oligonucleotide “

    x”

    by chemical or enzymatic ligation in each of the different reaction compartments, thereby generating a plurality of different oligonucleotide coding parts comprising “

    xa” and

    identifying “

    XA”

    ; and

    vi) making the plurality of different oligonucleotide coding parts comprising “

    xa”

    double stranded by a process comprising a polymerase extension reactionwherein the molecule parts “

    XA”

    are small, non-polymeric molecules with a molecular weight of less than 1000 Da.

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